三七总皂苷对大鼠乳腺癌变进程及miRNA-21表达影响的研究

Efficacy of PNS on the progression and miRNA-21 expression of rats with breast cancer

目的:通过观察三七总皂苷对二甲基苯并蒽(DMBA)诱导大鼠的乳腺组织病理学改变,观察其对乳腺癌变进程的影响及探讨其可能机制。方法:将34只SD雌性大鼠随机分为空白对照组(N组)、模型对照组(M组)、三苯氧胺组(T组)、三七低剂量组(S-L组)、三七中剂量组(S-M组)、三七高剂量组(S-H组),后五组均采用DMBA造模并分组干预,12周后将乳腺组织标本进行检测,比较不同组间乳腺组织病变情况、癌变率、miRNA-21的表达情况。结果:后五组大鼠乳腺组织均有不同程度的成瘤癌变,且T组、S-L组、S-M组、S-H组大鼠乳腺癌发率较M组明显降低(P<0.05),五组分别是36.67%,40.00%,26.67%,16.67%与91.67%。S-M组、S-H组miRNA-21的表达显著低于T组、S-L组和M组,略高于N组的大鼠正常乳腺组织。结论:三七总皂苷在一定程度上能够减慢癌变进程,且疗效与剂量成正比,可能与miRNA-21下调相关。

Objective： To observe the pathological changes of the mammary gland in rats by PNS, and to observe the effect of PNS on the progression of breast cancer and explore its possible mechanism. Methods： 34 SD female rats were randomly divided into the N group, M group, T Group, S-L group, S-M group and S-H group. The following five groups were modeled by DMBA and intervened. After 12 weeks, the breast tissue specimens were tested. The pathological changes, canceration rate and miRNA-21 expression of breast tissue were compared. Results： In the following five groups, there were different degrees of tumor carcinogenesis in the rat breast tissue. The incidence of breast cancer in the T group, S-L group, S-M group and S-H group were 36.67%, 40.00%, 26.67%, 16.67% respectively, and lower than 91.67% in M group（P〈0.05）. The miRNA-21 expression in the S-M group and S-H group were significantly lower than the T group, S-L group and M group, and slightly higher than the N group. Conclusion： PNS can slow down the process of carcinogenesis to some extent. The effect of PNS is directly proportional to the dosage, which may be related to the miRNA-21 reduction.