自噬蛋白p62在宫颈鳞癌组织中的表达及其与高危型人乳头瘤病毒感染相关性

Expression of autophagy protein p62 in cervical squamous carcinoma and its relativity with high risk human papilloma virus infection

目的探讨p62蛋白的表达与宫颈鳞状细胞癌发生发展的关系及其与高危型人乳头瘤病毒(HRHPV)感染的相关性。方法收集我院2011—2014年手术切除后保存的石蜡标本,宫颈鳞癌组织56例,正常宫颈组织20例,宫颈高级别鳞状上皮内瘤变组织34例,统计56例宫颈鳞癌患者HR-HPV的感染情况。采用免疫组织化学(SP)法检测p62蛋白在以上标本中的表达情况。用SPSS 13.0统计学软件分析p62蛋白在正常子宫颈上皮、宫颈上皮内瘤变及宫颈鳞癌组织中的表达差异,研究p62蛋白在宫颈鳞癌组织中的表达与宫颈鳞癌的临床特征之间的关系以及p62蛋白表达与宫颈鳞癌患者HR-HPV感染的相关性。结果 p62蛋白主要在宫颈鳞癌组织的细胞质及细胞核中表达,以细胞质中为主。在正常宫颈组织、宫颈高级别鳞状上皮内瘤变组织及宫颈鳞癌组织中自噬蛋白p62的阳性表达率分别为25%(5/20)、26%(9/34)、86%(48/56)。正常宫颈组织及宫颈上皮内瘤变组织中p62蛋白的阳性细胞的比例明显低于宫颈鳞癌组织(P<0.01)。自噬蛋白p62在正常宫颈组与宫颈上皮内瘤变组之间的表达差异无统计学意义,在正常宫颈组与宫颈鳞癌组之间p62蛋白的表达差异有统计学意义(P<0.05),宫颈上皮内瘤变组与宫颈鳞癌组之间p62蛋白的表达差异有统计学意义(P<0.05)。p62蛋白在伴有淋巴结转移的宫颈鳞癌组织中的阳性表达率为97%(32/33),在不伴淋巴结转移的宫颈鳞癌组织中的阳性表达率为70%(16/23),其高表达与淋巴结转移相关(P=0.037)。p62蛋白在伴HR-HPV感染的宫颈鳞癌组织中的阳性表达率为93%(42/45),在不伴HR-HPV感染的宫颈鳞癌组织中的阳性表达率为55%(6/11),2组比较差异有统计学意义(P=0.005)。p62蛋白的表达与宫颈鳞癌患者的年龄、临床分期、组织分化及肿瘤大小无相关性(P>0.05)。结论自噬蛋白p62蛋白在宫颈鳞癌组织中的高表达与淋巴结转移相关,与HR-HPV感染有关。推测p62蛋白的高表达可能与宫颈鳞癌的发生发展及预后有一定关系。

Objective To research the relationship of p62 protein with cervical squamous cell carcinoma′s occurrence and development and its relativity with high risk human papilloma virus（HR-HPV）infection. Methods CCollected56 cases of cervical squamous cell carcinoma, 20 cases of normal cervical and 34 cases of cervical intraepithelial neoplasis tissue from my hospital. Counted up HR-HPV infection status of 56 patients with cervical squamous carcinoma. We used the Streptomyces avidin peroxidaselink method（SP） immunohistochemistry technique to detect the expression of p62 protein in specimens of these tissue samples. We adopted statistical software statistical product and service solutions（SPSS） 13.0 to analyze p62 protein in cervical intraepithelial neoplasia,normal cervical epithelium and cervical squamous carcinoma tissues of expression differences. To research the relationship of p62 protein expression in cervical sq-uamous carcinoma tissues with cervical squamous car-cinoma clinical characteristics and the correlation between p62 protein expression in cervical squamous carcinoma tissues and HR-HPV infection with cervical squamous carcinoma patients. Results Autophagy protein p62 mainiy expressed in cervical squamous cell cancer of the cell nuclear and cytoplasm. The positive expression rate of p62 was 25%（5/20）,26%（9/34） and 86%（48/56） respectively in normal cervical epithelium,cervical intrae-pithelial neoplasia and cervical squamous carcinoma tissues. The expression of p62 protein in cervical squamous cancer was significantly higher than CIN and normal cervical tissue（P〈0.01）. Expression differences between autophagy protein p62 in normal cervix and cervical intraepithelial neoplasia group are no statistical significance,between the group of normal cervix and cervical squamous carcinoma p62 protein expression differences have statistical significance（P〈0.05）,between cervical intraepithelial neoplasia and cervical squamous carcinoma group p62 protein expression differences have statistical significance（P〈0.05）. Protein p62 associated with lymph node metastasis of cervical squamous carcinoma tissues in the positive expression rate was 97%（32/33）,nothing to do with lymph node metastasis of cervical squamous carcinoma tissues in the positive expression rate was 70%（16/23）,its high expression is associated with lymph node metastasis（P=0.037）. P62 protein in the positive expression in cervical squamous carcinoma tissues of HR-HPV infection rate was 93%（42/45）,positive expression in cervical squamous carcinoma tissues of no HPV infection rate was 55%（6/11）. Two groups of comparison companying, the difference is statistically significant（P=0.005））.P62 protein expression is not correlated to cervical squamous carcinoma patient＇s age, clinical stage, tissue differentiation and tumor size（P〉0.05）. Conclusion High expression of p62 protein is associated with the transfer of cervical cancer and related to the infection of HR-HPV. Show p62 protein expression is associated with the occurrence,development and prognosis of cervical squamous cell carcinomas.