前列化浊丸对慢性非细菌性前列腺炎大鼠的干预机制研究

Interventional Mechanism of Qianlie Huazhuo Pill in Rats with Chronic Nonbacterial Prostatitis

目的观察前列化浊丸对慢性非细菌性前列腺炎(chronic nonbacterial prostatitis,CNP)模型大鼠白细胞介素2(interleukin 2,IL-2)、白细胞介素8(interleukin 8,IL-8)、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、白细胞介素10(interleukin 10,IL-10)的影响,并探讨前列化浊丸治疗CNP的作用机制。方法选取雄性15周龄、SPF级SD大鼠50只,将其随机分为空白对照组,模型组,前列化浊丸低、中、高剂量组,每组10只。采取去势加皮下注射苯甲酸雌二醇复制大鼠CNP模型。空白对照组每日予以生理盐水灌胃,前列化浊丸低、中、高剂量组每日分别给予0.85、1.7、3.4g/kg前列化浊丸溶液灌胃,各组连续灌胃30d。灌胃治疗30d后处死大鼠,取大鼠血清及前列腺组织,观察各组大鼠前列腺组织形态学变化,比较各组大鼠前列腺组织内白细胞数量,检测各组大鼠血清中IL-2、IL-8及前列腺组织中TNF-α、IL-10水平。结果与模型组比较,各治疗组大鼠前列腺炎性反应显著减轻,前列腺组织病变程度均有所改善。与空白对照组比较,模型组前列腺组织内白细胞数量显著增加(P<0.05);与模型组比较,前列化浊丸低、中、高剂量组前列腺组织内白细胞数量显著减少(P<0.05)。与空白对照组比较,模型组大鼠血清中IL-2和IL-8水平均显著上升(P<0.05);与模型组比较,前列化浊丸低、中、高剂量组大鼠血清中IL-2和IL-8水平均显著下降(P<0.05)。与空白对照组比较,模型组大鼠前列腺组织中TNF-α和IL-10水平均显著上升(P<0.05);与模型组比较,前列化浊丸低、中、高剂量组大鼠前列腺组织中TNF-α和IL-10水平均显著下降(P<0.05)。结论前列化浊丸治疗CNP的作用机制可能与抑制IL-2、IL-8、TNF-α表达有关。

Objective To investigate the effects of Qianlie Huazhuo Pill on interleukin-2 （IL-2）, interleukin-8 （IL-8）, tumor necrosis factor-α （TNF-α）, and interleukin-10 （IL-10） in a rat model of chronic nonbacterial prostatitis （CNP） and the mechanism of action of Qianlie Huazhuo Pill in the treatment of CNP. Methods A total of 50 specific pathogen-free male Sprague-Dawley rats aged 15 weeks were selected and randomly divided into blank control group, model group, and low-, middle-, and high-dose Qianlie Huazhuo Pill groups, with 10 rats in each group. Castration and subcutaneous injection of estradiol benzoate were performed to establish a rat model of CNP. The rats in the blank control group were given normal saline by gavage every day, and those in the low-, middle-, and high-dose Qianlie Huazhuo Pill groups were given the solution of Qianlie Huazhuo Pill by gavage at daily doses of 0.85 g/kg, 1.7 g/kg, and 3.4 g/kg, respectively; the course of treatment was 30 consecutive days for these groups. The rats were sacrificed after 30 days of treatment by gavage, and serum and prostate samples were collected. The histomorphological changes of the prostate were observed, the number of leukocytes in prostate tissue was compared between groups, and the serum levels of IL-2 and IL-8 and the levels of TNF-α and IL-10 in prostate tissue were measured for all groups. Results Compared with the model group, the treatment groups had significant improvements in inflammatory response of the prostate and the severity of prostatic lesion. Compared with the blank control group, the model group had a significant increase in the number of leukocytes in prostate tissue （P〈0.05）; compared with the model group, the low-, middle-, and high-dose Qianlie Huazhuo Pill groups had a significant reduction in the number of leukocytes in prostate tissue （P〈0.05）. Compared with the blank control group, the model group had significant increases in the serum levels of IL-2 and IL-8 （P〈0.05）; compared with the model group, the low-, middle-, and high-dose Qianlie Huazhuo Pill groups had significant reductions in the serum levels of IL-2 and IL-8 （P〈0.05）. Compared with the blank control group, the model group had significant increases in the levels of TNF-α and IL-10 in prostate tissue （P〈0.05）; compared with the model group, the low-, middle-, and high-dose Qianlie Huazhuo Pill groups had significant reductions in the levels of TNF-α and IL-10 in prostate tissue （P〈0.05）. Conclusion Qianlie Huazhuo Pill has a good clinical effect in the treatment of CNP, possibly by inhibiting the expression of IL-2, IL-8, and TNF-α.