罗非鱼酶解物矿物离子结合能力及其结合物抗氧化活性

Mineral ion binding activity of hydrolysates from Tilapia muscle and antioxidant activities of combinations

采用胰蛋白酶对罗非鱼肉进行酶解,研究酶解产物对Ca^(2+)、Fe^(2+)、Zn^(2+)的结合活性,比较了酶解产物与矿物离子结合后的抗氧化活性。结果表明:水解度为7.41%的酶解产物具有最高的矿物离子结合能力,Ca^(2+)的结合率为78.71%,Fe^(2+)的结合率为73.84%,Zn^(2+)的结合率为71.09%;同时,结合物显示了一定的抗氧化活性,Ca^(2+)结合物1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除率为77.35%,羟自由基清除率为32.51%,还原力为0.29,均显著高于Fe^(2+)、Zn^(2+)结合物(p<0.05);氨基酸组成成分分析结果表明:具有高矿物离子结合活性的罗非鱼酶解产物中谷氨酸、天冬氨酸、赖氨酸、丝氨酸含量较高。

The enzymatic hydrolysis from Tilapia meat treated by trypsin,mineral ion binding activities and the antioxidant activities were investigated. The results showed that the hydrolyzates（ DH = 7. 41） had the highest mineral ion binding activity. Its Ca^2＋ combination rate was 78. 71%,Fe^2＋ combination rate was 73. 84%,and Zn^2＋ combination rate was 71. 09%. The combinations exhibited some antioxidant capacity. The Ca^2＋ combinations scavenging rate of DPPH（ 1,1-diphenyl-2-picrylhydrazyl） radical and hydroxyl radical, as well as reducing power were 77. 35%,32. 51%,0. 29,respectively,which were significantly higher than those of Fe^2＋,Zn^2＋ combinations. In addition,the amino acid sequence analysis showed that the hydrolyzates with higher contents of Glu,Asp,Lys and Ser had the higher mineral ion binding activity.