枸杞多糖脂质体制备工艺

Liposome preparation technology of lycium barbarum polysaccharide

以大豆卵磷脂和胆固醇为膜材,采用薄膜分散水化法,以包合率为目标采用响应面法对枸杞多糖的脂质体制备工艺进行优化。通过单因素实验得出药脂比(枸杞多糖与膜材的质量比)、膜材比(大豆卵磷脂与胆固醇的质量比)、水化温度均对包合率有影响。在此基础上,根据Box-Benhnken中心组合方法设计3因素3水平的试验,以包合率为响应值,做响应面分析。得到最佳工艺条件为:药脂比为1∶32.15、膜材比为3.84∶1、水化温度为43.26℃。此条件下预测包合率为70.77%,实际包合率为70.10%,误差值为0.95%,实验结果表明此方法包合率较高且易于控制。同时扫描电镜下观察到水化后枸杞多糖脂质体形态特征。

In this experiment,soybean lecithin and cholesterol was used as the membrane materials,through film dispersion and hydration method,using entrapment efficiency as the index,The response surface method was applied to optimize the lycium barbarum polysaccharide liposome preparation technology. The ratio of drug to lipid ratio,membrane material,water temperature were all major factors. Based on the test of three factors and three levels of Box-Benhnken center combination method design,The best conditions were as follows： drug lipid ratio was 32. 15,the membrane material ratio was 3. 84,water temperature of 43. 26 ℃. Under the above conditions,the predicted Entrapment efficiency was 70. 77% and the actual entrapment efficiency was 70. 10%. The error was 0. 95%. The experimental results show that this method has high entrapment efficiency rate and was easy to control. The morphological characteristic of liposomes samples after hydration was reveled under scanning electron microscope.