富硒玉米肽对扑热息痛致小鼠肝损伤的防护作用

Protective Effects of Selenium-Enriched Corn Peptides on Liver Injury Induced by Acetaminophen in Mice

目的:研究富硒玉米肽(selenium-enriched corn peptides,Se CPs)对扑热息痛(acetaminophen,APAP)代谢相关酶的影响及其对肝损伤的防护作用。方法:利用碱醇液提取玉米蛋白,经酶解和超滤制备分子质量小于5 k Da的混合玉米肽。建立扑热息痛致小鼠肝损伤模型,测定小鼠肝脏指数、血清谷草转氨酶(aspartate aminotransferase,AST)活力、肝脏中细胞色素P4502E1(cytochrome P4502E1,CYP2E1)、细胞色素P4501A2(cytochrome P4501A2,CYP1A2)、谷胱甘肽巯基转移酶(glutathione S-transferase,GST)、尿苷二磷酸葡萄糖醛酸转移酶(uridine diphosphate-dependent glycosyltransferase,UGT)质量浓度、磺基转移酶(sulfotransferase,SULT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力及还原型谷胱甘肽(glutathione,GSH)含量,苏木精-伊红(hematoxylin-eosin,HE)染色观察肝脏病理学变化。结果:与模型组相比,Se CPs组小鼠其肝脏中的CYP2E1、CYP1A2、UGT质量浓度及SULT活力显著降低(P<0.05),GSH含量、GST质量浓度及GSH-Px活力显著提高(P<0.05),肝脏指数和血清中AST活力明显降低,肝脏病理学情况显著改善。结论:Se CPs对过量扑热息痛致肝损伤的防护作用效果显著性优于亚硒酸钠+玉米肽,即有机硒与玉米肽有良好的协同保肝作用。

Objective： To investigate the effect of selenium-enriched corn peptides（Se CPs） on the enzyme activities related to the metabolism of acetaminophen and its protective effect on liver injury. Methods： Se-enriched corn proteins were extracted with a aqueous solution Na OH and alcohol, and then hydrolyzed by alcalase. Se CPs with a molecular weight less than 5 k Da were prepared by ultrafiltration. Liver injury was induced by intraperitoneal injection of acetaminophen in mice. Liver index, aspartate aminotransferase（AST） activity in serum, and the contents of cytochrome P4502 E1（CYP2 E1）, cytochrome P4501 A2（CYP1 A2）, glutathione S-transferase（GST）, uridine diphosphate-dependent glycosyltransferase（UGT） and glutathione（GSH）, the activities of sulfotransferase（SULT） and glutathione peroxidase（GSH-Px） in mice liver were determined. Hematoxylin-eosin staining was used to observe pathological liver changes. Results： Compared with the model group, the contents of CYP2 E1, CYP1 A2, UGT and SULT in the liver of mice treated with Se CPs were significantly decreased（P〈0.05）, while the contents of GSH and GST and the activity of GSH-Px were significantly increased. In addition, liver index and serum AST activity were significantly lowered and liver pathology was significantly improved. Conclusion： The protective effect of Se CPs on liver injury induced by excessive acetaminophen was better than that of combined sodium selenite and corn peptides（CPs ＋ Na2 Se O3）. Therefore, the combined use of organic selenium and corn peptides had a good synergistic effect on liver protection.