莲原花青素低聚体在模拟生理环境下对非酶糖化末端产物的抑制作用

Inhibitory Effect of Oligomeric Procyanidins of Lotus Seedpod on Advanced Glycation End Products Formation in Simulated Physiological Environment

为探究莲原花青素低聚体(oligomeric procyanidins of lotus seedpod,LSOPC)在模拟生理环境下对非酶糖化末端产物(advanced glycation end products,AGEs)的抑制作用,测定金属离子、高糖和高蛋白质量浓度条件下LSOPC对体外AGEs的抑制率,以及固定比例法评价LSOPC与表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)复配物对体外AGEs生成的抑制作用。结果表明:在模拟生理环境下,AGEs体外孵育最适反应时间为35 d,LSOPC的抑制效果达到最大值。不同质量浓度的金属离子(Cu^(2+)、Fe^(2+)、Zn^(2+)、Ca^(2+)、Mg^(2+)、Al^(3+)、Sn^(2+))对体外AGEs生成的影响各不相同,其中Cu^(2+)和Fe^(2+)在模拟体系中AGEs生成的促进作用最强,但和LSOPC一起有协同抑制作用;其他5种金属离子对AGEs生成都有不同程度的抑制或促进作用,但对LSOPC对AGEs的抑制作用几乎无影响。在高糖高蛋白反应体系中,发现LSOPC在高糖、高蛋白以及单独高蛋白情况下,抑制效果最差。LSOPC与EGCG复配有协同抑制AGEs效果,并且在LSOPC与EGCG质量浓度比为2∶1时最佳。

The objective of this work was to explore the inhibitory effect of oligomeric procyanidins of lotus seedpod（LSOPC）on the formation of advanced glycation end products（AGEs）in a simulated physiological environment.The percentage inhibition of AGEs by LSOPC in the presence of metal ions,high glucose and high protein concentration was determined and the inhibitory effect of LSOPC complexes with epigallocatechin gallate（EGCG）on the formation of AGEs was evaluated.The results showed that the optimal incubation time to obtain the highest inhibitory effect of LSOPC was 35 d.Inhibitory effects on AGEs formation varied depending on the type and concentration of metal irons added.Cu2＋and Fe2＋had the highest promoting effects on AGEs formation and could work together with LSOPC.Five other metal irons had different inhibitory or promoting effects on AGEs formation but hardly influenced the effect of LSOPC.We also found that LSOPC had the lowest inhibitory effect at high concentrations of both BSA and glucose or at high concentrations of BSA.Additionally,LSOPC in combination with EGCG showed a potent synergistic inhibitory effect and the optimal effect was obtained at a ratio of 2：1.