黑果腺肋花楸花色苷提取工艺优化及其抗氧化活性和组成鉴定

Optimization of Extraction of Anthocyanins from Berries of Aronia melanocarpa and Their Antioxidant Activity and Composition

目的:优化超声波辅助提取黑果腺肋花楸花色苷的条件,测定花色苷的抗氧化能力,鉴定黑果腺肋花楸花色苷提取物的组成成分。方法:采用响应面法优化花色苷提取条件,通过1,1-二苯基-2-三硝基苯肼自由基清除能力、2,2’-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐自由基清除能力及Fe3+还原能力方法评价其抗氧化能力,高效液相色谱-质谱联用法进行成分分析。结果表明:提取温度60℃、超声功率100 W、料液比1∶30(g/m L)、超声时间31 min、乙醇体积分数64%和pH 2.0时为最优提取条件,此时黑果腺肋花楸花色苷含量可达(3.61±0.01)mg/g。体外抗氧化实验表明,在相同质量浓度条件下,黑果腺肋花楸花色苷提取物的抗氧化活性明显高于VC。组分鉴定表明黑果腺肋花楸花色苷提取物中共有6种花色苷,其中矢车菊-己糖苷二聚体和矢车菊-3,5-二己糖苷为新检测出的2种花色苷。

This study aimed to optimize the ultrasonic-assisted extraction of anthocyanins from Aronia melanocarpaand to determine the antioxidant capacity and constituents of the obtained extract.The optimization was carried out by response surface methodology.The 1,1-diphenyl-2-picrylhydrazyl（DPPH） radical scavenging capacity,2,2＇-azinobis-（3-ethylbenzthiazoline-6-sulphonate） radical（ABTS＋·） scavenging capacity and Fe3＋ reducing ability of plasma（FRAP） assays were used to evaluate the antioxidant capacity.The anthocyanin components were analyzed by high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）.The results showed that the optimum extraction conditions were as follows：extraction temperature 60 ℃,ultrasonic power 100 W,solid-to-liquid 1：30（g/m L）,ultrasonic time 31 min,ethanol concentration 64%,and pH 2.0.Under these conditions,the content of anthocyanins was determined to be（3.61 ± 0.01） mg/g.The in vitro antioxidant activity of the anthocyanin extract was significantly higher than that of vitamin C at the same concentration.Six anthocyanins including two new compounds,dimer of cyanidin-3-hexoside and cyanidin-3,5-dihexoside,were detected in the extract.