高效液相色谱-串联质谱法检测鲜冻肉中违禁注水药物

Determination of Illegal Water-Retaining Drugs in Frozen Meat by UPLC-MS/MS

建立鲜冻肉中同时检测阿托品、利多卡因、普鲁卡因、山莨菪碱、东莨菪碱、沙丁胺醇6种违禁注水药物残留的高效液相色谱-串联质谱法。样品经磷酸盐缓冲液提取,HLB固相萃取小柱净化,采用多反应监测模式检测,外标法定量分析。结果表明:6种药物在0.25~50 ng/mL范围内线性关系良好,线性相关系数均大于0.99,检出限和定量限分别为0.5μg/kg和2.0μg/kg;回收率在78.07%~96.76%之间,精密度的相对标准偏差在0.4%~2.1%之间(n=6)。该方法前处理简单、快速、准确、灵敏,可以满足实际检测分析的需要。

An ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） method was developed for the determination of six illegal water-retaining drugs,atropine,lidocaine,procaine,anisodamine,scopolamine and salbutamol,in frozen meat.The samples were extracted with phosphate buffer,and the extract was cleaned up on an HLB cartridge.The analytes were detected in the multiple reaction monitoring mode and quantified by the external standard method.The results indicated that good linearity over the concentration range of 0.25–50 ng/mL was observed for all analytes with correlation coefficients greater than 0.99.The limits of detection（LOD） and quantification（LOQ） were 0.5 and 2.0 μg/kg,respectively.The average recoveries of the six drugs ranged from 78.07% to 96.76% with relative standard deviations（RSDs） of 0.4%–2.1%（n = 6）.The method is sensitive,simple,rapid and accurate and can meet the requirements of practical analysis.