部分型圆头精子症患者精子形态、DNA碎片指数和精液常规参数分析

The morphology,DNA fragment,and semen parameters analysis of patients with partial globozoospermia

目的:对部分圆头精子症患者的精子形态、DNA碎片指数和精液常规参数进行分析,研究DNA碎片指数与其他指标之间的相关性。方法:将2016年1月~2017年1月在解放军第113医院泌尿外科生殖中心进行不育症检查的155例患者纳入研究。按照是否患有部分型圆头精子症将患者分为观察组和对照组,观察组83例患者,对照组72例患者。检测形态正常精子率、精子总数、精子前向活动率(progressive motility,PR)、非前向活动率(non-progressive motility,NP)、不活动率(Immotile,Im)、精子DNA碎片指数(DNA fragment index,DFI)和核精蛋白色霉素3(Chromomycin A3,CMA3)染色阳性率(%)。结果:两组患者相比,观察组患者形态正常精子率、PR和NP均低于对照组患者,其差异均具有统计学意义(均P<0.05);观察组患者Im、DFI和CMA3阳性率均高于对照组患者,其差异均具有统计学意义(均P<0.05)。观察组患者DFI与Im及CMA3之间均存在正相关关系(均P<0.05);与形态正常精子率、PR、NP均呈负相关(均P<0.05)。结论:DFI是反应部分型圆头精子症患者精子功能的重要标志物,与精子染色质异常密切相关。

Objective： To analyze the morphology,DNA fragment,and semen parameters of patients with partial globozoospermia,and explore the correlation between DNA fragment index（ DFI） and other indexes.Methods： 155 patients in the Reproductive Center of Urology Department of the 113 Hospital of People＇s Liberation Army for male infertility from Jan 2016 to Jan 2017,were enrolled and divided into the observation group（ n = 83）and the control group（ n = 72） according whether be diagnosed as partial globozoospermia. Normal morphology,total sperm quantity,progressive motility（ PR）,non-progressive motility（ NP）,immotile（ Im）,DFI and chromomycin A3（ CMA3） positive sperm rate were analyzed. Results： The ratio of normal morphology,PR,and NP of the observation group were significantly lower than the control group,with statistically significant difference（ all P〈0. 05）. Im,DFI,and CMA3 of the observation group were significantly higher than the control group,with statistically significant difference（ all P〈0. 05）. DFI was positively correlated with Im and CMA3（ all P〈0. 05）,and negatively correlated with normal morphology,PR,and NP（ all P〈0. 05）. Conclusions： DFI is an important biomarker to reflect the sperm function of patients with partial globozoospermia,and closely correlated to the chromatin abnormality.