溴吡斯的明与糖皮质激素对寻常型天疱疮鼠模型棘层松解影响的比较研究

Comparative study of effects of pyridostigmine bromide and glucocorticord on acantholysis in a mouse model of pemphigus vulgaris

目的:建立寻常型天疱疮(PV)鼠模型,并比较溴吡斯的明与糖皮质激素对PV棘层松解的影响。方法 :将55只Balb/c新生鼠分为4组。模型组(15只):皮下注射PV患者血清,建立PV鼠模型;溴吡斯的明组(15只)和甲泼尼龙组(15只)除注射患者血清外,分别注射溴吡斯的明和甲泼尼龙;对照组(10只)皮下注射正常人血清作为对照。从临床表现、皮损组织病理和直接免疫荧光3方面评价溴吡斯的明与糖皮质激素对PV鼠模型棘层松解的影响。结果:模型组表现为棘层松解;溴吡斯的明组和甲泼尼龙组棘层松解程度均较模型组低(P<0.05);甲泼尼龙组棘层松解程度低于溴吡斯的明组(P<0.05);对照组小鼠皮肤未发生明显变化。结论:抗胆碱酯酶药及糖皮质激素均可减轻PV鼠模型的棘层松解程度,而抗胆碱酯酶药减轻棘层松解的作用低于糖皮质激素。

Objective： To compare the effects of pyridostigmine bromide and glucocorticoid on acantholysis in a mouse model of pemphigus vulgaris（PV）. Methods： Mouse model of PV was established by subcutaneous injection of serum from PV patients to neonatal Balb/e mice. Afterward 15 mice were injected with either pyridostigmine bromide or methylprednisolone group. Fifteen mice injected with serum from PV patients alone and 10 mice injected with normal serum served as controls. The effects of pyfidostigmine bromide and glucocorticoid on acantholysis were evaluated according to the clinical manifesta tions, histopathology and direct immunofluorescence. Results： The extent of aeantholysis in mice injected with either pyridostigmine bromide or methylprednisolone was less than that injected with patients＇ serum alone（P〈0.05）. In addition, the extent of acantholysis in methylprednisolone-treated group was less than that in pyridostigmine bromide-treated group（P〈0.05）. In contrast, normal serum did not alter the morphology of mouse skin. Conclusions： Both anticholinesterase agents and glucocorticolds can reduce acantholysis in PV mouse model, while the anticholinesterase drugs are less effective.