金黄地鼠高脂血症动物模型的应用研究

APPLICATION RESEARCH OF HYPERLIPIDEMIC ANIMAL MODEL OF GOLDEN HAMSTER

目的 :建立金黄地鼠高脂血症模型。方法 :按血浆 TC将 6～ 8周龄雄性金黄地鼠随机分成普通组和高脂组 ,分别饲以胆固醇含量为 0 .0 2 2 %和 0 .2 %、脂肪含量为 4 %和 2 0 %的饲料。每周采血一次 ,测定血浆 TC、TG。结果 :第三至四周动物 TC、TG达到较稳定水平 ,与普通组比较 ,高脂组动物 TC升高了近 2倍 ,TG升高近 1倍。结论 :建立金黄地鼠高脂血症模型所用饲料胆固醇含量低、造模时间短 ,是研究人类脂代谢的理想模型之一。

Objective:To create a new hyperlipidemic animal model using male golden hamsters.Methods:Sixteen animals aging from 6 to 8 weeks were divided into two groups randomly depending on TC.The objects in control group were fed with 0.022% cholesterol and 4% fat diet,and those in experimental group were fed with 0.2% cholesterol and 20% fat diet.Serum TC,TG was measured once a week.Results:By the end of 4th and 5th week,TC,TG in experimental group animals increased by 187% and 79% respectively compared with control group and reached a stable high level.Conclusion:The golden hamster hyperlipidemic animal model was created successfully using diet relatively lower in cholesterol and fat within a short period.This is an ideal model in lipid research.