加味补中益气汤治疗Ⅲ度大鼠内痔分子生物学作用机制分析

Analysis on Molecular Biological Mechanism of Using Modified Buzhong Yiqi Decoction in the Treatment of Degree Ⅲ Internal Hemorrhoids

目的:分析加味补中益气汤治疗Ⅲ度内痔分子生物学作用机制。方法:选取健康SD大鼠20只,平均体重208.16±20.49g,三月龄,随机将大鼠分成两组,观察组(10只)、对照组(10只),肛周注射75%冰醋酸法造模,而后观察组大鼠使用加味补中益气汤灌胃,对照组无菌蒸馏水灌胃,观察大鼠便血及痔核脱出评分改变状况,取大鼠肛垫组织标本,Western blot法检测组织内Fibulin-5蛋白表达状况,Real-time PCR法检测组织内Fibulin-5mRNA表达状况,检测处死时大鼠血清D-二聚体(D-D)、纤维蛋白原(FIB)含量及部分活血凝血活酶时间(APTT)、血浆凝血酶原时间(PT)和凝血酶原时间(TT)状况。结果:灌药14天后,观察组大鼠痔核脱出及便血评分显著降低,较造模后差异有统计学意义(P<0.05);观察组大鼠Fibulin-5mRNA与Fibulin-5蛋白表达均高于对照组,差异有统计学意义(P<0.05);观察组大鼠血清FIB、D-D含量及TT、PT时间较对照组显著降低,APTT时间较对照组升高,差异均有统计学意义(P<0.05)。结论:加味补中益气汤可改善大鼠痔相近动物模型出血情况,缓解肛周局部溃疡及水肿症状。

Objective： Analysis on molecular biological mechanism of using Buzhong Yiqi Decoction in the treatment of degree III internal hemorrhoids. Methods： 20 healthy SD rats,average weight 208. 16± 20. 49 g,march age,were randomly divided into two groups,observation group（ 10 rats） and controlled group（ 10 rats）,perianal injection of 75% acetic acid model,then observed rats to use Buzhong Yiqi Decoctioin orally,the controlleded group of sterile distilled water,rats in the stool and prolapse of hemorrhoids score change,specimens of anal cushion tissue of rats,the expression of Fibulin-5 protein in western detection by blot,expression of Fibulin-5 mRNA Real-time PCR method in tissue detection,detection of serum D-D in rats were sacrificed two dimers（ D-D）,fibrinogen（ FIB） content and blood thromboplastin time（ APTT）,prothrombin time（ PT） and prothrombin time（ TT） status. Results： Irrigation after 14 days treatment,observation of prolapse of hemorrhoids and hematochezia rats score decreased significantly,the difference was statistically significant compared with the model（ P〈0. 05）; The observation group rats Fibulin-5 mRNA and Fibulin-5 protein expression were higher than the controlled group,the difference was statistically significant（ P〈0. 05）; The observation group rats serum FIB,D-D content and TT,PT was significantly lower than the controlled group,APTT increased compared with the controlled group,the differences were statistically significant（ P〈0. 05）. Conclusion： Modified Buzhong Yiqi Decoction can improve the bleeding of the animal model of similar hemorrhoids in rats and relieve the local ulcers and edema symptoms in the perianal.