回直肠侧侧吻合分流术对慢传输型便秘大鼠血清和结肠组织匀浆中5-HT含量的影响

Effects of the ileo-rectal anastomosis bypass procedure on 5-HT in the blood plasma of the rats with slow transit constipation

目的完成慢传输型便秘(STC)大鼠回肠直肠吻合分流手术,观察该术后STC大鼠血清、结肠中五羟色胺(5-HT)值的变化。方法 72只SD大鼠,造模成功后(造模过程中死亡5只)完成回肠直肠吻合分流术,最终对照组10只,模型组12只,恢复组10只,手术组35只(术中死亡3只);测定并比较各组大鼠的血清、结肠中5-HT的含量。采用SPSS17.0软件进行数据分析。所有的统计检验均采用双侧检验,大鼠血清、结肠组织中5-HT值的变化采用±s表示,组间比较采用两独立样本的t检验,P<0.05为差异有统计学意义。结果血清中5-HT:与对照组相比,模型组大鼠血清5-HT水平显著增高(P=0.002<0.01);恢复组与模型组对比无明显差异(P=0.351>0.05),但明显高于对照组(P=0.001<0.01)。术后1个月,手术组与恢复组差异无统计学意义(P=0.171>0.05)。结肠组织匀浆中5-HT:与正常对照组相比,模型组、恢复组、手术组大鼠结肠组织匀浆中5-HT均显著升高(P<0.01);恢复组与模型组差异无统计学意义(P=0.263>0.05),手术后大鼠结肠组织中5-HT较恢复组增高。结论 STC大鼠血清及结肠组织中5-HT值明显增高,回直肠吻合分流术能改善STC大鼠的排便情况,但术后结肠中5-HT仍增高,结肠功能未能恢复。

Objective To investigate the influence of the ileo-rectal anastomosis bypass procedure on on the 5-HT in the blood plasma of therats with STC. Methods 72 SD rats were completed after the model was successful（ 5 dead in the process of modeling） and completed the ileo rectal anastomosis operation. The final control group was 10,12 in the model group,10 in the recovery group and 35 in the operation group（3 in the operation）,and the content of 5-HT in the serum and colon of the rats was measured and compared. Statistical analyses were performed using SPSS17. 0 software. Two-sided test was used in all statistical tests. The value of 5-HT in the serum and colon were presented by x± s. Comparisons between two groups were made by independent t test. Only differences of P 〈0. 05 were considered to be significant. Result 5-HT in serum： Compared with normal control group,5-HT in model group was increased considerably（ P = 0. 002 0. 01）. There was no significant difference between operation group and recovery group（ P = 0. 171 0. 05）,but operation group was still higher than model group. 5-HT in colon：Compared with normal control group,5-HT was significant increased in model group,recovery group,and operation group（ P 〈0. 01）; While there was no significant deviation between control group and recovery group. Operation group was higher than recovery group（ P = 0. 001 0. 05）. Conclusion 5-HT was significantly increased in the rats of STC model constipation. The ileo-rectal anastomosis bypass procedure do a great improvement in symptom of STC rats,but 5-HT in rats after surgery is still increased,The role of5-HT in gastrointestinal motility should be further studied.