宁夏无果枸杞芽水提物促眠效果研究

The effect of Ningxia fruitless lycium sprout aqueous extract on improving sleep

目的观察无果枸杞芽水提物(FLSAE)促眠效果。方法选取成年2月龄C57BL/6J小鼠,采用戊巴比妥钠协同FLSAE致小鼠睡眠的方法。将FLSAE按照5 mg/kg、10 mg/kg和20 mg/kg的浓度溶解在蒸馏水中,以0.2 mL/10(g·d),灌胃给药,持续11 d,对照组给予等量蒸馏水,观察小鼠入睡潜伏时间和睡眠持续时间。在上述研究的基础上,找出最佳药物浓度,检测各时间点小鼠的睡眠潜伏期和睡眠时间,筛选出最佳起效时间点。结果与对照组比较,FLSAE各浓度组小鼠睡眠潜伏期均缩短(P<0.05),睡眠时间均延长(P<0.05);以中浓度组(10 mg/kg)延长小鼠睡眠时间最为显著(P<0.05)。以10 mg/kg给药,给药第1天,与对照组比较,小鼠睡眠潜伏期有缩短(P<0.05)、睡眠时间有延长(P<0.05);至灌胃给药5 d时小鼠睡眠时间延长最显著,与对照组比较,P<0.05,与其他给药时间点比较,P<0.05;至灌胃11 d小鼠的睡眠潜伏期和睡眠时间与对照组比较,P<0.05。结论 FLSAE能够促进小鼠的睡眠,其中10 mg/kg组灌胃5 d促进小鼠睡眠效果最佳。

Objective To observe the effect of Ningxia Fruitless Lycium Sprout Extracts（FLSAE） on sleep in mice.Methods 2 months C57 BL/6 J mice wer eselected,the method of sleep in mice induced by pentobarbital sodium synergistic FLSAE.The mice in FLSAE groups weregivenbygavage,once a day for 11 days,FLSAE was dissolved in distilled water according to the concentration of 5 mg/kg,10 mg/kg and 20 mg/kg,with 0. 2 mL/10 g·d,and the mice in the control group were given bygavage purified water,sleep latency time and sleeping time of mice were observed. Based on the above result,choose 10 mg/kg FLSAE concentration group,analyzedsleeping latency and sleeping time of different groups,selected the bestreacting time and maintaining time. Results Compared with the control group,the sleep duration of mice were prolonged（P〈0.05） and the sleep latency was shortened（P〈0.05） in each FLSAE group. In comparison between the FLSAE groups,the sleeping timewas prolonged most significantly in 10 mg/kg group（P〈0.05）;compared with the control group,the sleep duration of mice was prolonged（P〈0.05） and the sleep latency was shortened（P〈0.05）from 1 day in FLSAE group; till 5 days,the sleep duration were prolong edsignificantly（P〈0.05）; the sleep latency and sleep duration of 11 days in FLSAE group were also different from in the control group（P〈0.05）. Conclusion FLSAE can improve sleep of mice; after gavaging FLSAE 10 mg/kg and continued 5 days the effect onimproving sleep is the best.