内皮抑素衍化30肽对HepG2细胞增殖活性和迁移能力的影响

Effect of Endostatin Derived 30 Peptide on Proliferation and Migration of HepG2 Cells

目的研究人内皮抑素衍化的30肽对肝癌HepG2细胞增殖和迁移能力的影响。方法人工合成人内皮抑素N末端30个氨基酸残基的编码序列(该多肽链为将原RGIRGAD更改为RGDRGD的30肽),连接到质粒pTYB2中,再转化至大肠埃希菌BL21(DE3)中表达,WST-1法检测30肽对HepG2细胞增殖活性的影响和transwell实验观察30肽对HepG2细胞迁移能力的影响。结果成功纯化30肽,30肽能够有效抑制HepG2细胞的增殖和迁移能力,且改抑制作用呈时间剂量依赖性。结论内皮抑素30肽能有效抑制HepG2细胞的增殖和迁移,其在临床上有望成为肝癌的治疗手段。

Objective This paper tries to investigate the effect of endostatin-derived 30 peptide on the proliferation and migration of HepG2 cells. Methods The coding sequence of the 30 amino acid residues of synthetic human endostatin,which is a 30-mer peptide that changed the original RGIRGAD to RGDRGD, was ligated into plasmid pTYB2 and transformed into Escherichia coli BL21（DE3） The effect of 30 peptide on the proliferation of HepG2 cells was detected by WST-1 assay and the effect of 30 peptide on the migration of HepG2 cells was observed by transwell assay. Results HepG2 cell viability and migration was significantly inhibited in a time-and dose-dependent response by peptide 30 of endostatin. Conclusion Endostatin 30 peptide can effectively inhibit HepG2 cell proliferation and migration, which is expected to become a clinical treatment of liver cancer.