摘要
[目的]研究补骨脂苷(PO)和异补骨脂苷(IPO)与牛血清白蛋白(BSA)的相互作用。[方法]通过紫外吸收光谱法、荧光淬灭光谱法、同步荧光光谱法、三维荧光光谱法分析PO和IPO对牛血清白蛋白的淬灭机制、结合常数、结合位点数及构象影响。[结果]PO和IPO对牛血清白蛋白产生了静态淬灭作用,两种药物与BSA有较弱的结合,在BSA上的结合位点均为位点Ⅱ,并且对BSA上色氨酸氨基酸残(Trp)基微环境产生了微弱的影响。[结论]借助光谱法揭示了PO和IPO与BSA相互作用的机制。
[Objective] To study of on the interaction between BSA and psoralenoside(PO) as well as isopsoralenoside(IPO). [Methods]Using the methods of UV absorption spectroscopy, fluorescence quenching spectroscopy, synchronous fluorescence spectroscopy and three-dimensional fluorescence spectroscopy quenching mechanism, binding constants, binding sites, conformational changes of PO as well as IPO to the BSA were analyzed. [Results] PO and IPO bound weakly to BSA with a static quenching process, which cause light changes in the polarity environment around the chromophore of tryptophan. The binding sites of the two drugs on BSA were Site II.[Conclusion] The mechanism of interaction between PO and IPO with BSA was revealed through the spectroscopy study.
作者
郭家鼎
郝佳
李梦荣
王誉程
刘二伟
GUO Jiading;HAO Jia;LI Mengrong;WANG Yucheng;LIU Erwei(Institute of Traditional Chinese Medicine Research,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae,Tianjin 300193,China)
出处
《天津中医药》
CAS
2018年第6期460-465,共6页
Tianjin Journal of Traditional Chinese Medicine
基金
天津市科技创新体系及条件平台建设项目(14TXZYJC00440)
天津市应用基础与前沿技术研究计划一般项目(15JCYBJC29300)
关键词
补骨脂苷
异补骨脂苷
紫外吸收光谱法
荧光光谱法
psoralenoside
isopsoralenoside
UV absorption spectroscopy study
fluorescence spectroscopy study