乳酸菌降解亚硝酸盐条件的优化

Optimization of the Degradation Conditions of Nitrite by Lactobacillus

[目的]研究Lyoflora V_3乳杆菌对亚硝酸盐降解的影响,优化其降解条件。[方法]探讨了乳酸菌降解亚硝酸盐的影响因素,主要包括溶液pH、接种量、Na Cl含量和培养时间。采用L_9( 3~4)正交试验对整体工艺进行试验条件优化。[结果]影响乳酸菌降解亚硝酸盐的各因素次序为pH>接种量>Na Cl含量>培养时间,最佳降解条件:pH为3.0,接种量为5%,Na Cl含量为9%,培养时间为72 h。在最佳降解条件下,乳酸菌降解亚硝酸盐的降解率可达98.50%。[结论]该研究可为乳酸菌发酵工艺中亚硝酸盐的控制提供参考。

[Objective]To study the effect of Lactobacillus Lyoflora V3 on nitrite degradation and optimize its degradation conditions. [Method]Factors influencing degradation of nitrite by Lactobacillus were discussed,including pH,inoculation quantity,Na Cl content and inocubation time.L9（ 34） Orthogonal design was adopted to optimize the overall process.[Result]The results show that the order of factors affecting the degradation of nitrite by Lactobacillus is： pH inoculation quantity Na Cl content inocubation time. The best conditions were pH 3. 0,inoculation quantity5%,NaCl content 9%,inocubation time 72 h. Under the best degradation conditions,the degradation rate of lactic acid bacteria can reach 98.50%.[Conclusion]The study can provide a reference for the control of nitrite in the fermentation process of lactic acid bacteria.