摘要
[目的]构建一种新型DNA电化学传感器,并用于花椰菜花叶病毒35S启动子片段检测。[方法]通过使用氨基化多壁碳纳米管对玻碳电极进行修饰,利用氨基化多壁碳纳米管对硫堇-金纳米粒子-DNA纳米复合物的吸附固定得到用于识别目标DNA的捕获界面,并将亲和素-辣根过氧化物酶修饰的金纳米粒子作为结构末端的信号分子,完成夹心结构传感器组装。试验采用循环伏安法对辣根过氧化物酶催化过氧化氢得到的还原电流进行测试。[结果]该传感器可实现对目标DNA定量检测,线性范围为1×10-18~1×10^(-11)mol/L,检测限低至6.95×10^(-20)mol/L,可特异性识别错配序列,并且表现出良好的重现性和稳定性。[结论]该传感器在转基因食品的检测方面有良好的应用前景。
[Objective] The research aimed to construct a DNA electrochemical sensor to detect the Cauliflower Mosaic Virus 35 S promoter( Ca MV35 S). [Methods]We choosed avidin-horseradish peroxidase-gold nanoparticles( Au NPs-HRP-SA) and thionine-gold nanoparticlesDNA nanocomposite( Thi-Au NPs-DNA) as signal amplification molecule to construct the DNA electrochemical sensor. We used multi-walled carbon nanotubes functionalized by amino( NH3^-MWCNTs) as the bottom layer to immobilize Thi-Au NPs-DNA which could identify target DNA and Au NPs-HRP-SA as the terminal signal amplifying molecule. The hybridization reaction was monitored by cyclic voltammetry upon HRP as an electrochemical indicator and H2O2 as enzyme substrate. [Result] The sensor could achieve the goal of quantitative detection of t DNA and obtain a linear range from 1 × 10^(-18) mol/L to 1 × 10^(-11) mol/L with the detection limits of 6. 95 × 10^(-20) mol/L( S/N = 3). The sensor also show good application prospect with good selectivity,repeatability and stability. [Conclusion]The sensing method has a good prospect in the detection of genetically modified foods.
作者
刘亚倩
刘克勤
高娇娜
操小栋
叶永康
LIU Ya-qian;LIU Ke-qin;GAO Jiao-na(School of Food Science and Engineering,Hefei University of Technology,Hefei,Anhui23000)
出处
《安徽农业科学》
CAS
2018年第17期183-186,共4页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(31772099)
安徽省自然科学基金项目(1508085MC47)