心脑苏颗粒(无糖型)中4种成分在大鼠血浆中的药动学行为

Pharmacokinetic behaviors of four constituents in sugar-free Xinnaosu Granules in rat plasma

目的考察心脑苏颗粒(无糖型)中4种成分在大鼠血浆中的药动学行为。方法大鼠灌胃给予该药物0.5%CMC-Na混悬液(3 g/kg)后取血,UHPLC-MS/MS法测定血药浓度。然后,DAS2.0软件计算药动学参数。结果丹参酮ⅡA、丹酚酸B、人参皂苷Rg_1、三七皂苷R_1的血药浓度-时间曲线符合二室模型,其t1/2分别为(1.68±0.56)、(4.13±0.87)、(3.62±0.87)、(9.77±3.12)h,T_(max)分别为(0.51±0.19)、(1±0)、(6±0)、(4.00±1.09)h,C_(max)分别为(0.42±0.08)、(0.17±0.02)、(0.46±0.11)、(0.41±0.12)mg/L。结论心脑苏颗粒(无糖型)中4种成分的生物利用度均较高。

AIM To investigate the pharmacokinetic behaviors of four constituents in sugar-free Xinnaosu Granules in rat plasma. METHODS Rats intragastrically administered with the 0. 5% CMC-Na suspension of this drug（ 3 g/kg） had their blood collected for the determination of plasma concentration by UHPLC-MS/MS,after which pharmacokinetic parameters were calculated by DAS2. 0 software. RESULTS The plasma concentration-time curves for tanshinoneⅡA,salvianolic acid B,ginsenoside Rg1,notoginsenoside R1 accorded with two compartment model,with the t1/2 values of（ 1. 68 ± 0. 56）,（ 4. 13 ± 0. 87）,（ 3. 62 ± 0. 87）,（ 9. 77 ± 3. 12） h,Tmaxvalues of（ 0. 51 ± 0. 19）,（ 1 ± 0）,（ 6 ± 0）,（ 4. 00 ± 1. 09） h,Cmaxvalues of（ 0. 42 ± 0. 08）,（ 0. 17 ±0. 02）,（ 0. 46 ± 0. 11）,（ 0. 41 ± 0. 12） mg/L,respectively. CONCLUSION All the four constituents in sugar-free Xinnaosu Granules demonstrate high bioavailabilities.