翼首草化学成分及其神经保护活性

Chemical constituents from Pterocephalus hookeri and their neuroprotection activities

目的研究翼首草Pterocephalus hookeri(C.B.Clarke)Hoeck的化学成分及神经保护活性。方法翼首草95%乙醇提取物的分析采用Sephadex LH-20、DM-130大孔树脂、硅胶、HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。通过构建DAT:EGFP转基因斑马鱼帕金森疾病模型研究其神经保护活性。结果从中分离得到9个化合物,分别鉴定为大花双参苷A(1)、茶茱萸苷(2)、林生续断苷Ⅰ(3)、二甲氧基-林生续断苷Ⅲ(4)、林生续断苷Ⅲ(5)、laciniatosideⅠ-7-dibutyl acetal(6)、laciniatosideⅠ(7)、laciniatosideⅡ(8)、林生续断苷Ⅳ(9)。首次发现其中的含双环烯醚萜正丁醇活性部位具有对神经元有抗氧化方面的神经保护活性。结论化合物4为首次从该植物中分离得到,翼首草对神经元有保护活性。

AIM To study the chemical constituents from Pterocephalus hookeri（ C. B. Clarke） Hoeck and their neuroprotection activities. METHODS The 95% ethanol extract from P. hookeri was isolated and purified by Sephadex LH-20,DM-130 macroporous resin,silica and HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data. The neuroprotection activities were studied by establishment of Parkinson＇s disease model of genetically modified zebrafish. RESULTS Nine compounds were isolated and identified as triplostoside A（ 1）,cantleyoside（ 2）,sylvestroside Ⅰ（ 3）,sylvestroside Ⅲ dimethyl acetal（ 4）,sylvestroside Ⅲ（ 5）,laciniatoside Ⅰ-7-dibutyl acetal（ 6）,laciniatoside Ⅰ（ 7）,laciniatoside Ⅱ（ 8）,sylvestroside Ⅳ（ 9）. The n-Bu OH fraction of P. hookeri had good performance on neuroprotection in hydrogen peroxide model. CONCLUSION Compound 4 is isolated from this plant for the first time. P. hookeri has neuroprotection activities.