摘要
设计了一种RNA提取方法,解决了梨树RNA提取时容易得率低和降解、污染这一关键问题.采用本方法可以实现纯度高、含量大、完整性好的梨树总RNA提取,提高二代测序数据质量.
A method of RNA extraction is designed to solve the key problems of low yield,degradation and pollution in the pear tree RNA extraction process.Total RNA is obtained intacted with high purity and content by using this method,and be improved the data quality of the second generation sequencing is improved.
作者
申世刚
高燕霞
王峰山
周玉岩
谢颖
冯丽
郝光飞
SHEN Shigang;GAO Yanxia;WANG Fengshan;ZHOU Yuyan;XIE Ying;FENG Li;HAO Guangfei(College of Chemistry and Environmental Science,Hebei University,Baoding 071002,China;Hebei Provincial Institute for Drug Control,Shijiazhuang 050011,China;College of LifeSciences and Food Engineering,Hebei University of Engineering,Handan 056038,China)
出处
《河北大学学报（自然科学版）》
CAS
北大核心
2018年第4期392-395,共4页
Journal of Hebei University(Natural Science Edition)
基金
国家自然科学基金青年基金资助项目(3170158900)
关键词
梨树组织
RNA
提取方法
pear tree tissue
RNA
extraction method
