摘要
本文通过慢病毒介导siRNA干扰卵巢癌SKOV3细胞中心体相关蛋白55(CEP55)表达,以观察其对细胞增殖、凋亡、侵袭和迁移等生物学行为的影响。实验分为CEP55组(转染CEP55 siRNA慢病毒)、阴性对照组(转染不含CEP55 siRNA的慢病毒)和空白对照组(不处理)。结果显示:CEP55 siRNA慢病毒转染效率为(89.4±4.7)%,CEP55组CEP55的mRNA和蛋白表达显著降低(P<0.05),第12、24、36、48、72 h时的OD(570)值显著降低(P<0.05),细胞凋亡率[(23.7±6.9)%]显著增加(P<0.05),穿膜细胞数目[(11.3±4.1)/个]明显减少(P<0.05),细胞迁移距离[(245.8±33.7)μm]明显减小(P<0.05)。提示慢病毒介导siRNA干扰CEP55表达可显著抑制卵巢癌SKOV3细胞增殖、促进其凋亡、降低其侵袭和迁移能力。
In this paper,the expression of CEP55 in SKOV3 cells was interfered by lentiviral-mediated siRNA,and its effect on proliferation,apoptosis,invasion and migration of cells was observed. The experiment was divided into CEP55 group,negative control group and blank control group. The CEP55 group and negative control group was transfected with lentiviral-mediated CEP55 siRNA and lentivirus vector without CEP55 siRNA,respectively. The blank control group did not do any treatment. The results of this study： the transfection efficiency of lentiviral-mediated CEP55 siRNA was（ 89.4±4.7） %,the mRNA and protein expression of CEP55 in CEP55 group decreased significantly（ P 〈 0. 05）,the OD（ 570）value in CEP55 group decreased significantly at 12,24,36,48 and 72 h,the apoptosis rate in CEP55 group [（ 23. 7 ±6.9） %] increased significantly（ P〈0.05）,the number of crossed cells in CEP55 group（ 11.3± 4.1） reduced significantly（ P〈0.05）,the migration distance in CEP55 group [（ 245.8±33.7） μm]reduced obviously（ P〈0.05）. The results of this study suggested that lentiviral-mediated CEP55 siRNA can significantly inhibit the proliferation of SKOV3 cells,promote its apoptosis,and reduce its invasion and migration.
作者
陈春莹
程丽琴
付艳霞
CHEN Chunying;CHENG Liqin;FU Yanxia(Department of Gynaecology and obstetris,The Eighth Affiliated Hospital,Sunyat-sen University,Shenzhen 518000,Guangdong,China)
出处
《中南医学科学杂志》
CAS
2018年第4期358-361,364,共5页
Medical Science Journal of Central South China
基金
深圳市科技创新委员会科研项目资助(项目编号201774106)
