无患子皂苷对人肝癌细胞HepG2增殖与凋亡的影响

Proliferation and Apoptosis Effects of Sapindus-saponin on Human Hepatocellular Carcinoma HepG2 Cells

为了探究无患子皂苷对人肝癌细胞Hep G2增殖和凋亡的影响。本研究采用不同浓度的无患子皂苷对Hep G2细胞进行处理,分别利用MTT法、流式细胞术、qPCR的方法检测细胞增殖、周期和凋亡情况以及NF-κB和Caspase3的表达情况。结果发现25μg/m L^100μg/m L的无患子皂苷分别作用12、24和36 h后,显微镜下发现细胞形态发生明显变化,MTT法检测发现细胞的增殖受到明显的抑制。无患子皂苷作用24 h后流式细胞术检测出现明显的凋亡峰,细胞周期阻滞在G0/G1期。qPCR检测发现随着皂苷浓度的提高,Caspase3的表达上调,而NF-κB的表达下调。以上结果说明无患子皂苷能够抑制人肝癌细胞HepG2的增殖并诱导其凋亡。

To investigate the effect of sapindus-saponin on the proliferation of HepG2 cell lines. HepG2 cell lines were treated by different doses of sapindus-saponin. HepG2 cell viability were evaluated by MTT assay,cell change in morphology was observed by microscope; Fluorescence flow cytometry（ FCM） was applied to detect cell cycle and cell apoptosis; q PCR was used to detect the levels of NF-κB and Caspase3. The growth of human hepatocellular carcinoma HepG2 Cells were inhibited after Sapindus-saponin（ 25-100 μg/m L） treated for 12 h,24 h and 36 h. Meanwhile,morphological changes of cells were obvious. FCM assay indicated that apoptosis peak appeared evidently and HepG2 Cells were atrested in G0/Glphase; the Caspase3 expression were up regulated,and the NF-κB expression were down regulate with the sapindus-saponin concentration increased. In summary,Sapindus-saponin could inhibit the proliferation of HepG2 Cells and induce their apoptosis.