褪黑素减轻大鼠颈动脉球囊损伤后的内膜增生

Melatonin inhibits the intima hyperplasia in rat carotid arteries after balloon injury

目的探讨褪黑素能否够减轻大鼠颈动脉球囊损伤后的内膜增生。方法将20只雄性大鼠随机分为对照组、褪黑素组(5 mg/kg)、球囊损伤组(建立颈动脉球囊损伤模型)和褪黑素干预组。14 d后取大鼠的颈动脉,弹力蛋白染色并计算各组大鼠颈动脉的内膜面积与中膜面积的比值(i/m);Western blot检测颈动脉内NF-κB、IL-1β、IL-6和TNF-α的表达。结果球囊损伤能够明显诱导大鼠颈动脉i/m值的增加(P<0.01);褪黑素干预组i/m值明显回降(P<0.01);球囊损伤组颈动脉的NF-κB、IL-1β、IL-6和TNF-α的表达明显高于对照组及褪黑素组(P<0.01);褪黑素干预组NF-κB、IL-1β、IL-6和TNF-α的表达明显回降(P<0.05)。结论球囊损伤能够诱导大鼠颈动脉的炎性反应及内膜增生,褪黑素能通过抑制NF-κB介导的炎性反应减轻大鼠颈动脉球囊损伤后的内膜增生。

Objective To investigate the effect of melatonin on intima hyperplasia in rat carotid arteries after balloon injury. Methods Twenty male sprague-dawley rats were randomized into control,5 mg/kg melatonin injected,balloon injured,and balloon injured with melatonin injected. An established balloon-induced carotid artery injury was performed. Carotid arteries were harvested 14 days later. The ratio of intima to media（ i/m） was measured. Western blot was used to detected the expression of NF-κB,IL-1β,IL-6 and TNF-α. Results The balloon injury could significantly induce the increasing of i/m（ P〈0. 01） and the expression of NF-κB,IL-1β,IL-6 and TNF-α（ P〈0. 01） in rat carotid arteries. Melatonin could minimize this effect caused by balloon injury. Conclusions Melatonin may inhibit intima hyperplasia in rat carotid arteries after balloon injury through alleviatting the inflammation mediated by NF-κB.