人血高密度脂蛋白注射液对高胆固醇饲料所致新西兰白兔动脉粥样硬化病变的作用及与洛伐他汀的比较(英文)

Effects of Human High Density Lipoprotein Injection on Atherosclerotic Lesions in New Zealand White Rabbits Fed with Cholesterol-Rich Diet and the Comparison with Lovastatin

目的 研究人血高密度脂蛋白 (HDL)注射液对喂高胆固醇饲料家兔主动脉粥样指纹病变的影响。方法 实验家兔给与 1%胆固醇饲料 8周。在此期间 ,HDL组每只家兔每周静脉注射 5 0mg人血HDL ;洛伐他汀组每只家兔每天皮下注射 10mg洛伐他汀 ;安慰剂组每只家兔每天静脉注射 2 0mL生理盐水。人血HDL注射液由清华紫光古汉生物制药集团有限公司生产并提供。结果 在试验期间 ,各组实验动物喂高胆固醇饲料后血脂水平均有升高。第八周后 ,实验动物主动脉内膜表面富脂粥样病变面积比分别为 :安慰剂组 32 .6 %± 2 1.7% (x±s,下同 ) ;HDL组 9.16 %± 7.87% ;洛伐他汀组 2 0 .8%± 13.1%。血清总胆固醇水平分别为 :安慰剂组 10 .0± 2 .30g L ;HDL组 2 .92± 1.41g L ;洛伐他汀组 3.74± 1.73g L。血清HDL胆固醇水平分别为 :安慰剂组 0 .43± 0 .12g L ;HDL组 0 .6 2± 0 .2 3g L ;洛伐他汀组 0 .2 3± 0 .14g L。HDL组血管壁胆固醇含量比安慰剂组和洛伐他汀组都明显降低 ,说明人血HDL具有比洛伐他汀更有效的抗动脉粥样硬化病变作用。结论 人血HDL能够有效地抑制喂胆固醇饲料家兔主动脉粥样病变的作用 ,并能够调节血脂 。

Aim The effects of in vivo administration of human high density lipoprotein (HDL) injection on the development of aortic streaks were studied in cholesterol-fed rabbits. Methods The rabbits received a 1% cholesterol-rich diet for 8 weeks. During this period, the HDL group was intravenously administered with 50 mg/week of HDL injection; the lovastatin group was administered with 10 mg/day of lovastatin via subcutaneous injection; the placebo group received normal saline (0.9% NaCl). The HDL injection was manufactured and provided by Tsinghua Unisplendour Guhan Bio-pharmaceutical Corporation Ltd. During the study, plasma lipid levels followed a similar profile in all groups to cholesterol-rich diet. Results At the completion of study, atherosclerotic-like lipid-rich lesions covered 32.6%±21.7% (x±s), of the intima aortic surface in the placebo group, 9.1%±7.8% in the HDL group, and 20.8%±13.1% in the lovastatin group. The levels of plasma total cholesterol were 10.05±2.30, 2.93±1.41, and 3.74±1.73 g/L in placebo, HDL, and lovastatin groups, respectively. The levels of plasma high density lipoprotein cholesterol (HDLC)were 0.43±0.12, 0.62±0.23, 0.23±0.14 g/L in placebo, HDL, and lovastatin groups, respectively. The value of total cholesterol deposited within vessel wall were significantly lower in the aortas of the HDL group than those in the placebo group and lovastatin group. Human HDL injection showed a more efficient inhibition of atherosclerotic lesions than lovastatin did. Conclusion The administration of human HDL injection to cholesterol-fed rabbits dramatically inhibits the extent of aortic fatty streaks, modifies plasma lipid, and lipid deposition in the arterial wall.