同型半胱氨酸对血管内皮细胞血凝素样氧化型低密度脂蛋白受体1 mRNA表达及氧化型低密度脂蛋白摄取的影响

Effects of Homocysteine on Lectin-Like Oxidized Low Density Lipoprotein Receptor-1 mRNA Expression and Uptake of Oxidized Low Density Lipoprotein in Cultured Human Umbilical Vein Endothelial Cells

为了研究同型半胱氨酸对血管内皮细胞血凝素样氧化型低密度脂蛋白受体 1mRNA表达及对氧化型低密度脂蛋白摄取的影响 ,分别在培养的人脐静脉内皮细胞中加入不同浓度的同型半胱氨酸孵育 48h后 ,利用逆转录—聚合酶链反应检测血凝素样氧化型低密度脂蛋白受体 1mRNA表达的改变 ;采用放射配基法检测细胞对氧化型低密度脂蛋白的摄取。结果发现 ,随着同型半胱氨酸浓度的增加 ,血凝素样氧化型低密度脂蛋白受体 1mRNA表达增加 ;同时对氧化型低密度脂蛋白的摄取率也增加。提示同型半胱氨酸能促进脐静脉血管内皮细胞血凝素样氧化型低密度脂蛋白受体 1mRNA的表达和对氧化型低密度脂蛋白的摄取 。

Aim To investigate the effects of homocysteine on the lectin-like oxidized low density lipoprotein receptor (LOX-1) mRNA expression and uptake of ox-LDL in cultured human umbilical vein endothelial cells (hUVEC). Methods Total RNA was extracted with Trizol reagent. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to quantify mRNA expression of LOX-1 genein hUVECs. ox-LDL was radioiodinated with 125 I to detect the uptake of ox-LDL by hUVEC. Results LOX-1 mRNA expression increased after cells were incubated with increasing concentration of homocycteine (0.1, 1, 10 mmol/L) for 48 hours. The uptake of ox-LDL by hUVEC increased at the same time. Conclusion Homocysteine increases the expression of LOX-1 mRNA in hUVEC and uptake of ox-LDL by them which may contribute to the proatherogenic potential of homocysteine.