清源调脂胶囊对高脂血症大鼠低密度脂蛋白受体基因表达的影响

Effect of Qing Yuan Tiao Zhi Capsule on Low Density Lipoprotein Receptor Gene Expression in Hyperlipemia Rats

从分子水平探讨清源调脂胶囊的降脂作用机理。将 2 7只Wistar大鼠随机分为正常组 (n =9)、高脂血症组 (n =9)和治疗组 (n =9)。正常组大鼠每日予以普通饲料常规喂养 ,高脂血症组和治疗组大鼠每日予以高脂饲料喂养 ,饮水不限 ,计 8周。其中 ,治疗组大鼠从造模一周末起 ,每日同步予以清源调脂胶囊 0 .96g 只 ,加水溶至 5mL ,分上下午两次灌胃。实验结束时 ,取各组大鼠肝组织为检测样本 ,进行总mRNA提取 ,cDNA合成 ,设内参照模板竞争法定量聚合酶链反应检测。结果发现 ,治疗组大鼠低密度脂蛋白受体基因表达水平比高脂血症组明显提高(P <0 .0 0 1)。结果提示 ,清源调脂胶囊能有效改善并提高实验性高脂血症大鼠低密度脂蛋白受体基因的表达 。

Aim To study the mechanism of Qing Yuan Tiao Zhi Capsule (QYTZC)decreasing blood-fat at the molecular level. Methods 27 Wistar rats were randomly divided into normal group (n=9), hyperlipemia group (n=9) and treatment group (n=9). In the experiment, all the rats from both hyperlipemia group and treatment group were daily fed with high fat feed for 8 days,with no limit to drinking except those from normal group, which were fed with ordinary feed. The rats in treatment group were given QYTZC (3 pellets for each, dissolved in water of 5 mL)by perfusion, twice a day after a week of the modeling. After the experiment, low density lipoprotein receptor (LDLR) mRNA was detected in the hepatic tissues of the rats from each group by reverse transcriptase-polymerase chain reaction (RT-PCR). Results QYTZC had a significant effect on LDLR gene expression of the rat (P<0.001). Conclusions QYTZC could increase LDLR gene expressin in rat hepatic tissues and, thus, decrease the level of blood-fat.