木薯MeRbohE基因的克隆及表达分析

Cloning and Expression Analysis of MeRbohE Gene in Cassava

从木薯中克隆了1个Rboh基因—Me Rboh E,该基因全长5 397 bp,开放阅读框2 775 bp,编码925个氨基酸,编码的蛋白分子量为104.8 KDa,理论等电点为8.90,蛋白结构具有典型的植物Rboh基因家族特征。q RT-PCR结果表明,Me Rboh E基因在木薯组培苗的根中表达量最高,茎中次之,叶中最低;在受到低温(4℃)、高盐(300 mmol·L-1Na Cl)和100 mmol·L-1ABA诱导后,表达量呈现不同程度的上调,表明该基因能应答非生物胁迫。

In this paper, we cloned a Rboh gene from cassava-Me Rboh E, the whole length of the gene is 5 397 bp,open reading frame is 2 775 bp, encoding 925 amino acids, the molecular weight of the protein is 104.8 KDa, and the theoretical isoelectric point of the protein is 8.90, the protein structure has typical plant Rboh gene family characteristics. The results of q RT-PCR showed that the expression of Me Rboh E gene was the highest in the roots,followed by the stems, and the lowest in the leaves; the expression of Me Rboh E gene was up-regulated after being induced by low temperature（4 ℃）, high salt（300 mmol·L-1 Na Cl） and 100 mmol·L-1 ABA. The results indicated that the gene could respond to biological stress and lay a foundation for elucidating the mechanism of cassava Rboh gene in stress resistance.