检测CEA和CEA mRNA指标诊断恶性腹水

Study on Detection of Carcinoembryonic Antigen mRNA and CEA in Specimens of Ascites to Diagnose Malignant Ascites

目的:评估检测癌胚抗原(CEA)和癌胚抗原信使核糖核酸(CEAmRNA)指标在诊断恶性腹水方面的应用价值。方法:研究对象包括恶性腹水组38例、良性腹水组32例,采集腹水为检测标本,应用酶联免疫吸附试验(ELISA)方法检测CEA、反转录套式聚合酶链反应(RT-NP-PCR)技术检测CEAmRNA。结果:CEA的阳性率为恶性腹水组52.6%(20/38)、良性腹水组6.3%(2/32);CEAmRNA的阳性率为恶性腹水组78.9%(30/38)、良性腹水组9.4%(3/32)。检测腹水标本CEAmRNA和CEA指标,诊断恶性腹水的特异性分别为CEA93.8%和CEAmRNA90.6%,敏感性分别为CEA52.6%和CEAmRNA78.9%。结论:在诊断恶性腹水方面,CEA和CEAmRNA指标的特异性均较高,但CEAmRNA指标的敏感性显著性高于CEA指标(P<0.01),更具有应用价值。

Objective:To evaluate the detection of carcinoembryonic antigen(CEA),carci-noem bronic antigen mRNA(CEAmRNA)in diagnosing malignant ascites.Methods:The CEA was detected by enzyme-linked immunosorbent assay(ELISA)and CEAmRNA was detected by re verse transcriptase-nested primers-polymerase chain reaction(RT-NP-PCR)in the specimens from38patients with malignant ascites and32patients with benign ascites.Results:The positive rates of CEA and CEAmRNA were52.6%(20/38)and70.9%(30/38)respectively in malignant ascites group,whereas those were6.3%(2/32)and9.4%(3/32)respectively in benign ascites group.The specificity rates of CEAmRNA and CEA were90.6%and93.8%respectively in diagnosing malig-nant ascites and the sensitivity rates of CEAmRNA and CEA were70.9%and52.6%re spectively.Conclusion:CEA and CEAmRNA are useful indexes for laboratory diagnosis of ma lignant ascites and the sensitivity of CEAmRNA is even more than CEA.(P<0.01)