甜荞热激转录因子基因家族的鉴定及生物信息学分析

Identification and bioinformatics analysis of heat shock factor family in buckwheat

利用Blastp比对程序对甜荞基因组数据库进行搜寻,共鉴定到23个甜荞HSF基因。基因结构分析显示,所有HSF基因都含有内含子,且大部分基因只含有1个内含子。蛋白序列多序列比对分析表明,23个HSF蛋白均含有氨基酸序列高度保守的DNA结合域(DNA–binding domain,DBD)和不保守的寡聚化结构域(oligomerization domain,OD)。蛋白保守基序分析表明,23个HSF蛋白共包含10个保守基序,基序长度为11~62个氨基酸,其中基序1、2和3代表DBD区。亚细胞定位分析表明,除Fe HSF13、Fe HSF14和Fe HSF18同时定位于细胞核和细胞质上,其余20个Fe HSF蛋白均定位于细胞核上。磷酸化位点分析表明,所有HSF蛋白均含有潜在的丝氨酸(Ser)和苏氨酸(Thr)磷酸化位点,部分HSF蛋白还含有酪氨酸(Tyr)磷酸化位点。系统发育分析表明,23个HSF基因可以分为A、B和C 3类,进一步细分为A1、A3、A4、A5、A6、A7、A9、B3、B4和C共10个亚类。

In the present study, a total of 23 HSF genes from common buckwheat genome were identified by using a systematic Blastp search. Gene structure analysis showed that 23 HSF genes contain intron and the intron number of most of these genes was one. Multiple alignment of protein sequences analysis indicated that all HSF proteins contain the conserved DNA–binding domain（DBD） and no conserved oligomerization domain（OD）. Conserved motif analysis found that 10 conserved motifs with a range of 11 to 62 amino acids existed in common buckwheat HSF proteins, and motif 1, 2 and 3 represent DBD domain. With the exception of Fe HSF13, Fe HSF14 and Fe HSF18 were located in both nucleus and cytoplasm, and the other 20 Fe HSF proteins were located in nucleus. Potential phosphorylation sites analysis indicated that all HSF proteins contain Ser and Thr phosphorylation site, and some proteins also include Tyr phosphorylation site. Phylogenetic analysis revealed that 23 HSF genes were divided into three classes（A, B and C）, and further subdivided into 10 subclasses（A1, A3, A4, A5, A6, A7, A9, B3, B4 and C）.