冷冻胁迫转金柑MLP2–1基因拟南芥的转录组测序和代谢通路

Transcriptome sequencing and metabolic pathway analysis of transgenic MLP2–1 gene in Arabidopsis under cold stress

利用农杆菌介导的花序浸润法,将金柑(Fortunella margarita(Lour.)Swingle)MLP2–1基因转入野生型拟南芥(Col–1),对转MLP2–1基因拟南芥在冷冻胁迫下目的基因的转录组数据进行分析。结果显示:转MLP2–1基因拟南芥的抗冻能力强于野生型拟南芥;转录组测序分析表明,转MLP2–1拟南芥与野生型拟南芥植株差异表达基因为1 422个,其中551个表达上调,871个表达下调;差异表达基因数目在GO数据库注释到1 208个,在KEGG数据库中注释到474个,在COG数据库中注释到603个;KEGG代谢通路富集结果表明,差异表达基因主要集中在植物激素与信号转导代谢通路。

In this study, miraculin–like protein 2（MLP2–1） gene of kumquat was cloned and transformed into wild–type Arabidopsis（Col–1） by Agrobacterium–mediated infiltration. The transcriptional data of transgenic Arabidopsis under colds tress was analyzed, which revealed the mechanism of MLP2–1 gene in Arabidopsis. The results showed that transgenic Arabidopsis had higher resistance than wild–type Arabidopsis under cold stress; comparing with the wild–type Arabidopsis, transcriptome analysis showed that 551 genes were up–regulated and 871 genes were down–regulated in total 1 422 differently expressed genes. Among of them, 474 annotations was from KEGG, 1 208 from GO and 603 from COG after functional annotation. KEGG analysis showed that differentially expressed genes mainly involved in the plant hormones signal transduction pathway, which will provided a foundation for further studying MLP2–1 functions of kumquat.