绿茶儿茶素对于DMBA诱发大鼠乳腺癌前增生的抑制作用

Inhibitory Effect of GTC to Breast Hyperplasia of Rat Induced by DMBA

目的:探讨GTC(Greenteacatechins,绿茶儿茶素)对于DMBA(9,10-Dimethylbenz-1,2-anthrancene,二甲基苯蒽)诱发的大鼠乳腺癌前增生的抑制作用。方法:42只7周龄雌性SD大鼠,随机分为A(空白对照)、B(DMBA灌胃)、C(GTC灌胃)、D(DMBA/GTC灌胃)4组。于实验开始日,B组和D组的大鼠一次性DMBA油溶液灌胃(7mg/100g体重);自实验开始日起,C组和D组的大鼠每日GTC水溶液灌胃1次(80mg/次)。4组大鼠均自由摄食基础饲料和饮用自来水,至实验结束。光镜下(100倍)计量各组大鼠乳腺导管的芽体、实团、乳头状增生和核分裂象等4项增生指标,并计算其平均值(x±s)。结果:1)B组的芽体、实团、芽体+实团、乳头状增生和核分裂象均显著地高于与其对照的A组;2)D组的实团、芽体+实团显著高于与其对照的C组,实团、乳头状增生较C组趋低;3)D组的芽体、实团、芽体+实团和乳头状增生显著低于B组,核分裂象在两组之间没有显著性差异;4)C组的各项增生指标均低于A组,但无显著性差异。结论:持续GTC水溶液灌胃可明显抑制DMBA诱导的大鼠乳腺的癌前增生。

Objective:To illustrate the inhibitory effect of GTC(green tea catechins)to the development of hyperplastic lesion in rat mammary gland induced by DMBA.Methods:A total of forty-two 7-week-old female SD rats was divided into4groups:group A(blank control),B(DM-BA gavage),C(GTC gavage)and D(DMBA/GTC gavage).,.At the beginning of experiment,group B and D received a7mg /100g bodyweight intragastric dose of DMBA,at the same time,group C and D received an intragastric dose of GTC(80mg /rat/day).The GTC-gavage was administered until the end of experiment.All rats got the basic fodder and tap water freely.Results:1)The buds,solid bodies,buds+solid bodies papilloid hyperplasia and mitosis in group B were signifi-cantly more than those in group A;2)Buds and buds+solid bodies in group D were significantly more than those in group C,and solid bodies and papilloid hyperplasia in group D were also,ore than those in group C,but not significantly.3)Buds,solid bodies,buds+solid bodies and papil-loid hyperplasia in group D were significantly less than those in group B,but the mitosis between these two groups showed no significance.4)All the four hyperplastic lesions in group C were less than those in group A,but not significant.Conclusion:Constant GTC-gavage can inhibit the de-velopment of hyperplastic lesions in duced by DMBA in SD rats effec tively