超声微波协同萃取枸杞黄酮的工艺研究

Study on the Technology of Ultrasonic Microwave Synergistic Extraction of Flavonoids in Medlar

采用超声微波协同萃取技术对枸杞中的总黄酮进行快速提取。在单因素实验的基础上设计正交实验对提取工艺条件进行优化,得到最佳条件为:提取温度50℃,提取液为60%的乙醇,料液比为1∶20,提取时间20min,在此条件下枸杞中黄酮的提取率可达1.98%。同时还将超声微波协同萃取法与传统冷凝回流法、超声波辅助提取法和微波提取法进行对比。结果表明,超声微波协同萃取法不仅简单高效,同时快速节能,非常适合枸杞中黄酮类化合物的提取。

Ultrasonic-microwave synergistic extraction technology was used to extract the total flavonoids from medlar. On the basis of single factor experiments,orthogonal experiments were used to optimize the extraction process. The optimum extraction conditions were as follows： extraction temperature 50℃,extraction liquid 60% ethanol,material-liquid ratio 1：20,extraction time20 min. In this condition,the extraction rate of flavonoids can reach 1. 98%. Simultaneously,the ultrasonic-microwave synergistic extraction method was compared with the traditional condensation reflux method,ultrasonic-assisted extraction method and microwave extraction method. The results show that the ultrasonic-microwave synergistic extraction method is not only simple and efficient,but also fast and energy-saving,and it is very suitable for the extraction of flavonoids from medlar.