摘要
目的:建立一种简单、快速、有效的方法测定黄芪中总黄酮含量。方法:在NaNO_3-Al(NO_3)_3-NaOH比色法辅助下,用紫外分光光度法测定黄芪中总黄酮的含量。结果:显色法最佳条件为5%NaNO_3-8%Al(NO_3)_3-5%NaOH,最大吸收波长为512nm,吸光度A与芦丁在0.015~0.6mg/mL范围内呈良好的线性关系(R^2=0.9995),线性方程为A=0.0741c+0.0005,总黄酮的回收率为97.6%,相对偏差(RSD)为2.47%。结论:该法简单准确,易操作,回收率高,可作为黄芪总黄酮含量测定的一种方法。
Objective：To establish a simple,rapid and effective method for determination of total flavonoids in Astragalus membranaceus.Methods：The content of total flavonoids in Astragalus membranaceus was determined by UV spectrophotometry with the aid of Na NO3-Al（NO3）3-Na OH colorimetric method.Results：The optimal conditions for chromogenic assay were 5%Na NO3-8%Al（NO3）3-5%Na OH,the maximum absorption wavelength was 512 nm,and the absorbance A was in a good linear relationship with rutin in the range of 0.015-0.6 mg/m L（R^2）.=0.9995）,the linear equation is A=0.0741c＋0.0005,the recovery of total flavonoids was 97.6%,and the relative deviation（RSD）was 2.47%.Conclusion：This method is simple and accurate,easy to operate,and has a high recovery rate.It can be used as a method for the determination of total flavonoids in Scutellaria baicalensis.
作者
陈凌霆
赵娅敏
齐燕姣
罗兴平
康淑荷
张丽丽
俞布仁
杨文亮
Chen Lingting 1,2 ,Zhao Yamin 1,2 , Qi Yanjiao 1,2 , Luo Xingping 1,2 ,Kang Shuhe 1,2 ,Zhang Lili 1,2 ,Yu Buren 3,Yang Wenliang 1,2(1.School of Chemical Engineering, Northwest Minzu University,Lanzhou 730000,China;2.Key Laboratory for Utility of Environment-Friendly Composite Materials and Biomass in Universities of Gansu Province,Northwest Minzu University,Lanzhou 730000, China;3.School of Electrical Engineering,Northwest Minzu University,Lanzhou 730000,Chin)
出处
《山东化工》
CAS
2018年第14期75-77,共3页
Shandong Chemical Industry
基金
中央高校基本科研业务专向资金项目(31920180003)
关键词
黄芪
总黄酮
比色法
紫外分光光度法
radix astragali
total flavonoids
colorimetric method
ultraviolet spectrophotometry
