摘要
研究采用琼脂平板扩散试验,以单核增生李斯特氏菌(Listeria monocytogene)54002为指示菌,从湖南传统发酵豆制品中筛选出1株对其具有明显抑制作用的芽孢杆菌(Bacillus sp.)HN-7,通过酸碱稳定性、酶敏感性、热稳定性及紫外稳定性试验,可初步推测该抑菌物质是脂肽。研究结果表明,菌株HN-7所产抗菌肽抑菌谱较宽,耐热、耐酸性强,对单增李斯特氏菌(Listeria monocytogenes)、金黄色葡萄球菌(Staphylococcus aureus)、大肠杆菌(Escherichia coli)、藤黄微球菌(Micrococus luteus),枯草芽孢杆菌(Bacillus subtilis)都具有抑制作用,并在121℃处理20 min、pH 2.0~12.0条件下仍具有抑菌活性。
Using agar plate diffusion tests, a strain with obvious inhibitory effect on Listeria monocytogenes 54002 was screened from traditional fermented soybean products in Hunan. After acid-base stability, enzyme sensitivity, thermal stability and ultraviolet stability tests, the results showed that the antibacterial substance was preliminarily speculated as lipopeptide. The antimicrobial peptide produced by strain HN-7 had a broad spectrum of inhibition and was resistant to heat and acidity. It had inhibitory effect on L. monocytogenes, Staphylococcus aureus, Escherichia coli, Micrococus luteus and Bacillus subtilis, and still had antibacterial activity after treatment at 121 ℃ for 20 min at pH 2.0-12.0.
作者
黄承敏
王蓉蓉
肖茜
刘成国
周辉
HUANG Chengmin1,2, WANG Rongrong1,2, XIAO Qian1,2, LIU Chengguo1,2, ZHOU Hui1,2(1.College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2.Hunan Provincial Key Laboratory of Food Science and Biotechnology, Changsha 410128, Chin)
出处
《中国酿造》
CAS
北大核心
2018年第7期31-34,共4页
China Brewing
基金
国家自然基金项目(31571811
31601525)
湖南省教育厅科学研究项目(16K043)
关键词
抗菌肽
芽孢杆菌
筛选
抗菌活性
antimicrobial peptides
Bacillus
screening
antibacterial activity
