基于对氨基苯硼酸修饰磁性纳米粒子及丝网印刷技术测定糖化血红蛋白

Determination of HbA1c Using a Screen Printed Electrode with 3-Aminophenylboronic Acid Modified Magnetic Nanoparticles

制备了对氨基苯硼酸修饰的磁性纳米粒子,其与血液中的糖化血红蛋白(Hb A1c)结合,通过网状玻璃态碳(RVC)电极的磁性区域后,可实现与未糖基化的血红蛋白分离。通过壳聚糖(CS)、正硅酸乙酯(TEOS)和碳纳米管构成的溶胶-凝胶膜修饰丝网印刷电极对血红蛋白进行电化学检测,从而建立了一种用于检测糖化血红蛋白的新方法。在0.10 V电位下,丝网印刷电极的电量与Hb A1c和Hb的浓度有较好的线性关系,检出限分别为6 mg/m L和0.05 mg/m L。该方法电极制作简单,有较好的重现性和稳定性,且能有效排除抗坏血酸(AA)、尿酸(UA)等的干扰,已成功应用于实际样品中Hb A1c的检测。

A novel method was developed for the detection of HbAlc using a screen printed electrode with 3-aminophenylboronic acid modified magnetic nanoparticles.The magnetic nanoparticles modified with 3-aminophenylboronic acid were prepared,which could bind with glyeosylated hemoglobin （HbA1c ） and be separated from hemoglobin when the sample is injected into the reticulated vitreous carbon （RVC ） flow cell.A screen printed electrode was fabricated by the reaction of chitosan （CS ）,tetraenthoxyl silica （TEOS ） and carbon nanotubes to form a sol-gel layer at the surface of the electrode.Based on this,a novel electrochemical method was developed for the detection of HbA1c.There were good relationships of electric charge at the screen printed electrode with concentrations of HbA1c and Hb under the potential of 0.10 V, and the limits of detection were 6 mg/mL and 0.05 mg/mL,respectively.With simple preparation,good reproducibility and stability,the method could effectively eliminate the interference of AA and UA.It was successfully applied in the detection of HbA1c in real samples.