干细胞相关技术治疗肝功能衰竭的基础与临床转化研究

Stem cell-related techniques for the treatment of liver failure: A basic and clinical translational study

肝功能衰竭病情凶险、进展迅速、预后较差,严重危害人民健康。肝移植是治疗肝衰竭最有效方法,但由于器官短缺,仅有少数患者能及时接受移植。干细胞相关技术(包括干细胞移植、生物人工肝等)因具有解毒、合成、代谢、分泌等功能,为这一问题的解决带来了希望。此项目在干细胞移植和生物人工肝2个领域取得了多项原创性成果,开创了肝衰竭治疗的新领域,具有显著的经济和社会效益。文中主要从3方面进行阐述:干细胞移植治疗肝功能衰竭的机制探讨和临床转化研究;骨髓间充质干细胞与猪肝细胞共培养体系的建立和基于共培养细胞的生物人工肝临床转化研究;人源化肝样诱导细胞的建立和基于人源化种子细胞的生物人工肝的临床转化研究。

Acute liver failure（ALF） is a severe clinical syndrome with rapid progression,poor prognosis and high mortality.Liver transplantation is the most effective option for the treatment of ALF,but only a few ALF patients can receive the donor liver in time due to the insufficient supply of the organ. Stem cell-related techniques,including stem cell transplantation,bioartificial liver,etc.,with their roles of detoxication,synthesis,metastasis and secretion,have brought some hope for the solution of this problem. This article presents an overview of the three aspects as follows： mechanisms and clinical translation of stem cell transplantation for ALF,establishment of the coculture system for mesenchymal stem cells with porcine hepatocytes and clinical translation of the coculture-based bioartificial liver,and establishment of human-induced hepatocytes and clinical translation of bioartificial liver based on human-induced seed cells.