新生儿科分离白假丝酵母菌多位点序列分型研究

Multilocus sequence typing of Candida albicans isolated from neonatal department

目的分析白假丝酵母菌多位点序列分型(MLST)与标本来源、药物敏感性之间的关系,探讨菌株间的亲缘关系及其分子流行病学特征。方法收集2015年4—5月某院新生儿科病房分离的白假丝酵母菌,采用试剂盒对菌株进行体外药物敏感性测试,并采用MLST方法对菌株进行分型,分析菌株间的同源性及分子流行病学特征。结果共分离15株白假丝酵母菌,来自4例患儿和1名医务人员,15株白假丝酵母菌经MLST获得3个序列型(ST),其中13株为ST1997,另外2株分别为ST1359和ST1933。经eBURST软件对ST分型进行聚类分析,ST1997菌株属于Group 13,ST1359菌株属于Group 1,ST1933菌株属于Group 20。同一患者外周血和外周静脉导管分离的白假丝酵母菌属于同一克隆株。结论新生儿科病房存在白假丝酵母菌血流感染的暴发,应积极制定相应的预防控制措施,加强对新生儿科病房的医院感染管理。

Objective To analyze the relationship between multilocus sequence typing（MLST） and sources of specimens as well as antimicrobial susceptibility of Candida albicans（C. albicans）, and explore the relationship among strains and their molecular epidemiological characteristics. MethodsC. albicans isolated from neonatal department in a hospital from April 2015 to May 2015 were collected, in vitro susceptibility testing was carried out by a kit, strains were typed by MLST, homology and molecular epidemiological characteristics of strains were analyzed. ResultsA total 15 C. albicans strains were isolated from 4 children and 1 health care worker, these strains were divided into 3 sequence types （ST） through MLST, 13 of which were ST 1997, and the other 2 were ST 1359 and ST 1933 respectively. Phylogenetic analysis of strains was carried out by eBURST software. ST 1997, ST 1359, and ST 1933 were in Group 13, Group 1,and Group 20 respectively, C. albicans isolated from peripheral blood and peripheral venous catheter of the same patient belonged to the same clone. ConclusionThere is an outbreak of C. albicans bloodstream infection in the neonatal department, corresponding preventive and control measures should be formulated to strengthen the management of healthcare associated infection in neonatal department.