马铃薯PAL基因的生物信息学初步分析

Bioinformatics Analysis of pal Gene in Potato

PAL是催化直接脱掉L-苯丙氨酸上的氨而生成反式桂皮酸的酶,是一个可把苯丙氨酸用于酚类化合物合成的酶。本文利用电子克隆技术获得一个马铃薯PAL基因的c DNA序列,运用多种生物信息学方法对其进行初步分析。从核酸序列到氨基酸序列,再到蛋白质的高级结构预测,获得了该基因的电子克隆全长。结果表明:该序列全长2529bp,共编码722个氨基酸,有多个限制性酶切位点,有38个磷酸化位点,属于亲水性氨基酸,无信号肽,无膜穿透性,无保守结构域。文末对下一步的实验验证和功能分析进行了展望,为进一步研究该基因在马铃薯特别是在抗病性的功能提供相关依据。

Pal is an enzyme that catalyzes the direct removal of ammonia from L-phenylalanine and the formation of trans-cinnamic acid. It is an enzyme that can use phenylalanine for the synthesis of pheno-lic compounds. In this paper, the cDNA sequence of a potato pal gene was obtained by using electronic cloning technique, and the cDNA sequence was preliminarily analyzed by various bioinformatics meth- ods. From nucleic acid sequence to amino acid sequence to advanced structure prediction of protein, the full length of electronic clone of this gene was obtained. The results showed that the sequence was 2529bp, encoding 722 amino acids, with multiple restriction endonuclease sites and 38 phosphorylation sites, which was hydrophilic. Amino acids, no signal peptide, no membrane penetration, no conserved domain. At the end of the paper, the future experimental verification and functional analysis were prospected, which provided the relevant basis for further study of the function of the gene in pota- to, especially in resistance to disease.