TLC-SERS联用快速同时检测食品中非法添加的碱性橙Ⅱ和酸性橙Ⅱ的研究

Study on the Determination of Basic Orange Ⅱ and Acid Orange Ⅱ in Food by TLC-SERS

建立了一种薄层色谱(TLC)与表面增强拉曼光谱(SERS)联用快速检测食品中非法添加的碱性橙Ⅱ和酸性橙Ⅱ的方法。采用薄层色谱法对样品提取液进行简单分离,并优化了薄层色谱分离条件;合成并优选出水相和有机相两类银溶胶,分别用作碱性橙Ⅱ和酸性橙Ⅱ拉曼信号增强基底,继而利用优选的银溶胶为增强基底对分离出的微量物质进行表面增强拉曼光谱检测,考察了检测时间,并确立了碱性橙Ⅱ和酸性橙Ⅱ检出限分别为1和2.5mg·L^(-1)。将该方法用于实际样品检测,成功实现了复杂食品基质中碱性橙Ⅱ和酸性橙Ⅱ的同时快速检测。该方法具有简便、快速、经济、专属性好等优势,为复杂食品基质中碱性橙Ⅱ和酸性橙Ⅱ的同时快速检测提供了新方案。

A method for the rapid detection of illegally added basic orange Ⅱ and acid orange Ⅱ in food was established by the combination of thin layer chromatography （TLC） and surface enhanced Raman spectroscopy （SERS）. TLC was used for the separation of sample extracts tentatively and the conditions were optimized. Two kinds of silver sols, aqueous phase and organic phase, were synthesized and used as the substrate to enhance Raman signal for basic orange Ⅱ and acid orange Ⅱ. SERS was used to detect isolated micro-substances and the time was investigated. The detection limits of basic orange Ⅱ and acid orange Ⅱ were 1 and 2.5 mg·L-1. Then, the method has been successfully used for rapid detection of basic orange and orange Ⅱ in the complex food matrix. The method has the advantages of simplicity, rapidity, economic and specificity, and will provide a new scheme for the simultaneous detection of basic orange Ⅱ and acid orange Ⅱ in food.