子宫内膜癌患者癌组织中核连蛋白-2表达与临床病理特征及预后的相关性分析

Expression of nucleobindin-2 protein in cancer tissue and its correlation with clinicopathological features and prognosis of patients with endometrial carcinoma

目的:探讨子宫内膜癌患者癌组织中核连蛋白-2(nucleobindin-2)表达与临床病理特征及预后的相关性。方法:选取本院妇科确诊的98例子宫内膜癌患者为子宫内膜癌组,同时设置正常子宫内膜组(24例)、复杂性增生病例组(36例)、子宫内膜不典型增生病例组(42例),采用酶联免疫吸附法及免疫组化方法测定各组nucleobindin-2表达。结果:复杂性增生组、子宫内膜不典型增生组、子宫内膜癌组nucleobindin-2表达水平高于正常子宫内膜组,且子宫内膜不典型增生组nucleobindin-2表达水平高于复杂性增生组,子宫内膜癌nucleobindin-2表达水平高于复杂性增生组、子宫内膜不典型增生组,差异均有统计学意义(t=10.231、11.343、13.432、12.453、10.871、11.094,均P<0.05);正常子宫内膜组、复杂性增生组、子宫内膜不典型增生组、子宫内膜癌组nucleobindin-2阳性率逐渐升高,且差异有统计学意义(χ~2=26.241,P<0.05)。子宫内膜癌患者nucleobindin-2表达与年龄相关性不明显,差异无统计学意义(χ~2=0.232,P>0.05);与病理分型、病理学分级、TMN分期、浸润深度、淋巴血管间隙浸润、淋巴结转移相关性明显,且子宫内膜样腺癌、病理学分期越高、TMN分期越高、浸润深度越深、有淋巴血管间隙浸润、有淋巴结转移,nucleobindin-2阳性表达率越高,差异有统计学意义(χ~2=6.112、20.312、10.121、24.152、7.723、8.734,P<0.05或P<0.01)。nucleobindin-2阴性组3年生存率及生存期均明显高于nucleobindin-2阳性组,差异有统计学意义(χ~2=14.697、12.993,P<0.05)。结论:nucleobindin-2在子宫内膜癌组织的表达量高于正常子宫内膜组织,nucleobindin-2在子宫内膜癌的发生发展过程中起促进作用;nucleobindin-2低表达的患者能获得较好的预后。

Objective： To investigate the expression of nucleobindin-2 protein in cancer tissue and its correlation with clinicopathological features and prognosis of patients with endometrial carcinoma. Methods： 98 cases with endometrial cancer gynecology diagnosed in our hospital were selected as the endometrial cancer group,and 24 cases with normal endometrium,36 cases with complex hyperplasia by surgical resection or outpatient cases,and 42 cases of endometrial atypical hyperplasia scraping were selected. The enzyme-linked immunosorbent assay and immunohistochemical method were used to detect the expression of nucleobindin-2 protein. Results： The expression level of nucleobindin-2 protein in complex hyperplasia group,atypical endometrial hyperplasia group and endometrial cancer group were higher than that of normal endometrium group,and the expression level of atypical endometrial hyperplasia group was higher than that in complex hyperplasia group,the expression of nucleobindin-2 protein in endometrial cancer group was higher than that of complex hyperplasia group and atypical endometrial hyperplasia group,the differences were statistically significant（ t = 10. 231,11. 343,13. 432,12. 453,10. 871,11. 094,P〈0. 05）.The nucleobindin-2 positive rate were gradually increased in the normal endometrium group,complex hyperplasia group,atypical endometrial cancer group and endometrial hyperplasia group,and the difference were statistically significant（ χ~2= 26. 241,P〈0. 01）. The relationship between the expression of nucleobindin-2 protein and age was not obvious（ χ~2= 0. 232,P〈0. 05）. The expression level of nucleobindin-2 protein was positively correlated with pathological grade,pathological types,TMN stage,invasion depth,lymph vascular space invasion,lymph node metastasis,and the higherpathological staging,the higher the stage of TMN,the depth of invasion deeper,lymph vascular space invasion,lymph node metastasis,the higherexpression rate of nucleobindin-2,and the difference were statistically significant（ χ~2= 6. 112,20. 312,10. 121,24. 152,7. 723,8. 734,P〈0. 05 or P〈0. 01）. The 3 years survival rate and survival time nucleobindin-2 in the negative group were significantly higher than that in the nucleobindin-2 positive group,the difference was statistically significant（ χ~2= 14. 697,12. 993,P〈0. 05）.Conclusion： The expression of nucleobindin-2 protein in endometrial carcinoma is higher than that in normal endometrial tissue. The nucleobindin-2 protein play an important role in the occurrence and development of endometrial cancer. The patients with low expression of nucleobindin-2 protein can obtain better prognosis.