糖尿病视网膜病变血清miR-146a与核转录因子-κB和VEGF的相关性

Correlation of serum miR-146a with nuclear factor-κB and vascular endothelial growth factor in diabetic retinopathy patients

目的:探讨糖尿病视网膜病变血清miR-146a与核转录因子-κB(NF-κB)和血管内皮生长因子(VEGF)的相关性。方法:选取2016-07/2017-12我院收治的2型糖尿病患者100例为研究对象,合并DR患者32例(DR组),未合并DR患者68例(T2DM组)。选取同期体检的健康志愿者30例作为对照组。采用real-time PCR检测血清miR-146a,采用酶联免疫法检测NF-κB和VEGF,分析miR-146a与NF-κB和VEGF的相关性。结果:与对照组比较,T2DM组和DR组Hb A1c升高(t=6.822、5.709,均P<0.001),空腹血糖(FBG)升高(t=8.889、7.923,均P<0.001),餐后2h血糖(2h PBG)升高(t=6.646、5.514,均P<0.001)。与T2DM组比较,DR组糖尿病病程较长(t=2.431,P=0.017)。与对照组比较,T2DM组和DR组血清miR-146a明显降低(t=3.967、7.169,均P<0.001),且DR组低于T2DM组(t=4.444,P<0.001)。与对照组比较,T2DM组和DR组血清NF-κB明显增加(t=6.063、14.851,均P<0.001),VEGF明显增加(t=7.613、12.943,均P<0.001);且DR组NF-κB和VEGF大于T2DM组(t=11.406、7.560,均P<0.001)。Pearson分析显示miR-146a与NF-κB和VEGF呈负相关(r=-0.503、-0.574,P<0.05)。结论:DR患者血清miR-146a明显降低,NF-κB和VEGF明显增加,miR-146a有可能通过介导炎症反应和血管增生参与DR的发病。

AIM： To investigate the correlation of serum miR-146 a with nuclear factor-κB（ NF-κB） and vascular endothelial growth factor（ VEGF） in diabetic retinopathy patients.METHODS： A total of 100 patients with T2DM treated in our hospital from July 2016 to December 2017 were assigned into T2DM patients with DR（ DR group,n =32）and T2DM patients without DR（ T2DM group,n = 68）.Thirty healthy volunteers were selected as control group.Real-time PCR was used to examine the expression of miR-146 a. Enzyme linked immunosorbent assay was used to detect the levels of NF-κB and VEGF. The correlation between miR-146 a and NF-κB and VEGF was analyzed.RESULTS： Compared with the control group,HbA1 c in T2DM group and DR group increased（ t = 6. 822,5. 709;P〈0. 001）,FBG increased（ t = 8. 889,7. 923; P〈0. 001）,2 hPBG increased（ t =6. 646,5. 514; P〈0. 001）. Compared with T2DM group,the duration of diabetes in DR group was longer（ t = 2. 431,P = 0. 017）. Compared with the control group,serum miR-146 a in T2DM and group DR significantly decreased（ t = 3. 967,7. 169; P〈0. 001）,and the DR group was lower than that in the T2DM group（ t =4. 444,P〈0. 001）. Compared with the control group,the serum NF-κB in the T2DM and DR group increased significantly（ t = 6. 063, 14. 851; P〈0. 001）, VEGF increased significantly（ t =7. 613,12. 943; P〈0. 001）,NF-κB and VEGF in DR group were larger than those in T2DM group（ t = 11. 406,7. 560; P〈0. 001）. Pearson analysis showed that miR-146 a was negatively correlated with NF-κB and VEGF（ r =-0. 503,-0. 574; P〈0. 05）.CONCLUSION： The serum miR-146 a in DR patients significantly decreased, the NF-κB and VEGF significantly increased. MiR-146 a may be involved in the pathogenesis of DR by mediating inflammatory reaction and vascular proliferation.