高氧液对兔全脑缺血再灌注损伤生化指标的影响

Protective effect of hyperoxia solution on brain injury resulting from ischemia and reperfusion in rabbits

目的 探讨高氧液对兔全脑缺血再灌注损伤的保护作用 .方法 18只家兔随机分 3组 (n=6 ) .用夹闭双侧颈总动脉加降压法建立全脑缺血模型 .A组再灌注时用平衡液 2 0m L· kg- 1 静脉推注 (对照组 ) ;在再灌注时用高氧液 2 0 m L·kg- 1 静脉推注 (治疗组 ) ;C组于实验前 3d用高氧液 2 0 m L·kg- 1静脉推注每日 1次 (预处理组 ) .各组在缺血前、缺血 2 0min和再灌注 4 5 min抽取颈内静脉血测定血浆一氧化氮(NO) ,超氧化物歧化酶 (SOD)和丙二醛 (MDA) .结果 再灌注后 B组和 C组 MDA含量明显低于 A组 (P<0 .0 1或 P<0 .0 5 ) ,B组和 C组血浆 SOD活性高于 A组 (P<0 .0 5 ) .B组和 C组血清 NO含量明显低于 A组 (P<0 .0 1或 P<0 .0 5 ) .结论 高氧液对神经元缺血损伤具有保护作用 .

AIM To investigate the protective effect of hyperoxia solution on the cerebral ischemia model. METHODS 18 domestic rabbits were randomly divided into three groups. In Group A the balance salt 20 mL·kg -1 (which wasreplaced by hyperoxia solution in Group A) was given intravenously to the animals after reperfusion. And in Group C hyperoxia solution 20 mL·kg -1 was infused intravenously everyday for 3 d before ischemia. The amount of NO and MDA as well as the activity of SOD in plasma were observed at different time points (before ischemia, after 20 min ischemia and after 45 min reperfusion). RESULTS 45 min after reperfusion, the amounts of NO and MDA in Group B and Group C were lower than those in Group A ( P <0.01 and P < 0.05 respectively). But the activity of SOD in Group B were significantly higher than in Group A ( P <0.05). CONCLUSION Hyperoxia solution can mitigate the cerebral ischemia and reperfusion injury.