地氟醚预处理对大鼠局灶性脑缺血损伤的保护作用

Neuroprotection induced by desflurane preconditioning against middle cerebral artery occlusion damage in rat

目的 探讨地氟醚预处理对脑局灶性缺血损伤的影响 .方法 采用大脑中动脉阻闭 (middle cerebral artery oc-clusion,MCAO)模型 ,将 30只雄性 SD大鼠随机分成 3组 :对照组 (C组 ,n=10 ) ,动物不接受任何处理 ;地氟醚组 (Des组 ,n=10 ) ,动物每日接受 1h的地氟醚预处理 (5 7m L· L- 1地氟醚 ,94 0 m L· L- 1 O2 ) ,连续 5 d;吸 O2 组 (O2 组 ,n=10 ) ,动物每日接受 1h的吸 O2 预处理 (94 0 m L· L- 1 O2 ,无地氟醚 ) ,连续 5 d.所有动物均在大脑中动脉阻闭 12 0 min后恢复再灌注 ,观察再灌注后 2 4 h动物神经行为学改变及脑梗死容积 .结果 再灌注后 2 4 h神经行为学评分 Des组明显低于 C组和 O2 组 (P<0 .0 1) ;2 4 h脑梗死容积 C组为 (2 76 .10±15 5 .6 3) m m3,O2 组为 (2 5 3.84± 174 .39) mm3,Des组 (5 9.5 6± 30 .37) m m3,Des组明显低于对照组 (P<0 .0 1) .结论 地氟醚预处理对大鼠局灶性脑缺血损伤具有保护作用 .

AIM To investigate if desflurane preconditioning induces ischemic tolerance against neuronal damage produced by middle cerebral artery occlusion (MCAO) in rats. METHODS Thirty male SD rats weighing 280~320 g were randomly divided into three groups: control group ( n =10), C group, without pretreatment; desflurane preconditioning group (Des group, n =10), which were pretreated with inhalation of 57 mL·L -1 desflurane in 940 mL·L -1 O 21 h per day for 5 d; and oxygen preconditioning group (O 2 group, n =10), which inhaled 940 mL·L -1 O 2 1 h per day for 5 d. Right MCAO (120 min) was induced by a 3 0 nylon thread with round tip inserted cranially into right internal carotid artery. The neurologic deficit score (NDS) was evaluated 24 h after reperfusion and the infarct volume was determined 24 h after reperfusion. RESULTS The NDS of the Des group was lower than the other two groups ( P <0.01). The infarct volumes of C, O 2 and Des groups were (276.10±155.63) mm 3, (253.84±174.39) mm 3 and (59.56±30.37) mm 3 respectively . The difference between Des and C groups was significant ( P <0.01). CONCLUSION Desflurane pretreatment was able to induce ischemic tolerance against neuronal damage produced by transient MCAO in rats.