肝外胆管癌中p73蛋白的表达及与p53蛋白的相关性

Expression of p73 and its relation to p53 in extrahepatic cholangiocarcinoma

目的 探讨基因 p73和 p5 3在肝外胆管癌 (ECHC)中的表达情况、相互关系及其临床病理意义 .方法 采用免疫组织化学 (SABC法 )对 4 1例肝外胆管癌及 10例胆总管囊肿病例 p73和 p5 3表达进行研究 .结果 p73基因阳性表达率为 4 1% (17/ 4 1) ,p5 3基因阳性表达率为 34% (14 / 4 1) ,14例中 10例 p73表达亦为阳性 ,其余 2 7例中未发现 p5 3基因突变 ,但其中 7例表达 p73.胆总管囊肿均为阴性 .经统计学分析 p73的表达与 ECHC的临床病理分级、分期无关 (P>0 .0 5 ) ;而 p73和 p5 3表达显著相关 (r=0 .5 2 ,P<0 .0 5 ) .结论 p73,p5 3基因在肝外胆管癌的发生发展过程中起重要作用 。

AIM To study p73 and p53 gene expressions in extrahepatic cholangiocarcinoma (ECHC) and their clinico pathological significance. METHODS Immunohistochemical method (SABC) was used to detect the p73 and p53 gene expressions in 41 cases of ECHC and 10 cases of choledochocyst. RESULTS Positive rates of p73 and p53 gene were 41%(17/41), 34%(14/41) resepectively. 10 of the 14 caseswere also stained positive for p73. In contrast, in the rest of the patients (27) without any detectable p53 mutation, 7 exhibited p73 immunostaining. Neither p73 nor p53 was detected in choledochocyst. There was no significant difference in expression of p73 between tumor grade and heson stage.But expression of p73 was significantly correlated to that of p53 ( r=0.52, P <0.05). CONCLUSION Both p73 and p53 genes play an important role in the development of ECHC. The expressions of p73 and p53 in ECHC are significantly correlated.