梯度浓度添加甘油提高冻融红细胞回收率的研究

Effect of Gradient Concentration of Glycerol on the Recovery of Freeze-Thawed Red Blood Cells

目的针对传统冰冻红细胞技术的缺点进行改良,建立一种快速、简便、高回收率的红细胞冰冻方法并形成由低到高的浓度。方法采用从低到高的浓度顺序快速添加法,将甘油保护剂分成3份,总体积不变。比较改良方法与传统方法冷冻前及解冻后的红细胞回收率、游离血红蛋白和甘油残留量等指标。结果改良方法的红细胞回收率(87.44±3.4)%,游离血红蛋白(0.65±0.31)g/L,甘油残留量(4.20±0.75)g/L;传统方法的检测结果相应为(81.77±3.2)%,(0.58±0.23)g/L和(2.50±0.34)g/L。两种方法红细胞回收率的差异具有统计学意义(P<0.05)。结论改良方法制备的红细胞,其各项指标均符合国家标准,且红细胞回收率比传统方法更高,红细胞质量得到进一步提高,对于保障输血安全、改善输血效果具有重要意义。

Objective To improvethe traditional frozen red blood cell technology, we have established a rapid, simple, high recovery rate of red blood cell freezing method. Methods The glycerol protective agent was divided into three parts, from low to high concentration and the total volume was constant. Three parts were added according to the sequence from low to high concentration. The recovery rate of red blood cells, free hemoglobin, and glycerol residue were compared between the traditional method and the improved method. Results Red blood cell recovery rate was （87.44± 8.4） %, free hemoglobin was （0.65±0.81） g/L, glycerin residue was （4.2±0.75） g/L in improved method. The control group for the corresponding results was （81.77±8.2） %, （0.58±0.28） g/Land （2.5±0.84） g/L. The red cell recovery rate of the two