血清营养素水平与肺炎患儿免疫细胞分化、炎症反应激活的相关性

The correlation of serum nutrient levels with immune cell differentiation and inflammatory response activation in children with pneumonia

目的:探讨血清营养素水平与肺炎患儿免疫细胞分化、炎症反应激活的相关性。方法:选择2015年4月~2017年8月间在本院接受治疗的肺炎患儿200例作为肺炎组,同期在本院进行疫苗接种的健康小儿100例作为正常对照组。对比两组小儿血清中营养素、Th1/Th2细胞因子、Th17/Treg细胞因子、炎症介质含量的差异。采用Pearson检验评估肺炎患儿血清营养素水平与病情严重程度的内在联系。结果:肺炎组患儿血清中Vit A、Fe、Zn的含量低于正常对照组小儿。两组小儿血清中Vit D、Ca、Mg水平的差异无统计学意义。肺炎组患儿血清中Th1细胞因子IL-2、TNF-β的含量低于正常对照组,Th2细胞因子IL-4、IL-5的含量高于正常对照组;Th17细胞因子IL-17、IL-21、IL-22的含量高于正常对照组,Treg细胞因子IL-10、TGF-β的含量低于正常对照组;炎症介质CRP、PCT、MCP-1的含量均显著高于正常对照组。Pearson检验发现,肺炎患儿血清中营养素Vit A、Fe、Zn的水平与免疫细胞分化、炎症反应程度直接相关。结论:肺炎患儿血清Vit A、Fe、Zn等营养素水平异常降低,具体水平与患儿病情严重程度直接相关。

Objective：To investigate the correlation of serum nutrient levels with immune cell differentiation and inflammatory response activation in children with pneumonia.Methods：200 children with pneumonia who were treated in our hospital between April 2015 and August 2017 were selected as the pneumonia group,and 100 healthy children who were vaccinated in this hospital during the same period were selected as the normal control group.The differences in serum levels of nutrients,Th1/Th2 cytokines,Th17/Treg cytokines and inflammatory mediators were compared between the two groups.Pearson test was used to assess the relationship between serum nutrient levels and disease severity.Results：Serum Vit A,Fe and Zn levels of pneumonia group were lower than those of control group.The differences in serum Vit D,Ca and Mg levels were not statistically significant between the two groups of children.Serum Th1 cytokines IL-2 and TNF-βcontents of pneumonia group were lower than those of control group whereas Th2 cytokines IL-4 and IL-5 contents were higher than those of control group;Th17 cytokines IL-17,IL-21 and IL-22 contents were higher than those of control group whereas Treg cytokines IL-10 and TGF-βcontents were lower than those of control group;inflammatory mediators CRP,PCT and MCP-1 contents were significantly higher than those of control group.Pearson test showed that serum nutrients Vit A,Fe and Zn levels in children with pneumonia were directly correlated with the degree of immune cell differentiation and inflammatory response.Conclusion：Serum Vit A,Fe,Zn and other nutrient levels abnormally decrease in children with pneumonia,and the specific level are directly correlated with the severity of the disease.