食管癌根治术前紫杉醇+顺铂化疗对肿瘤恶性程度的影响

Effect of paclitaxel+ cisplatin chemotherapy before radical operation for esophageal cancer on the malignant degree of tumor

目的:研究食管癌根治术前紫杉醇+顺铂化疗对肿瘤恶性程度的影响。方法:选择本院进行的食管癌根治术病例,回顾病史资料并根据是否进行术前紫杉醇+顺铂新辅助化疗分为实验组和对照组。手术后,留取两组患者的食管癌组织适量,抽提RNA后测定凋亡基因Smac、Caspase-9、p21、Spink8及侵袭基因ADAMTS5、CNTN1、β-catenin、MMP9、N-cadherin及血管新生分子TGF-β1、VEGF的mRNA表达量。结果:实验组患者手术切除食管癌组织中Smac、Caspase-9、p21、Spink8的mRNA表达量显著高于对照组,手术切除食管癌组织中ADAMTS5、CNTN1、β-catenin、MMP9、N-cadherin的mRNA表达量显著低于对照组,血清中TGF-β1、VEGF的含量以及手术切除食管癌组织中TGF-β1、VEGF的mRNA表达量显著低于对照组。结论:食管癌根治术前紫杉醇+顺铂化疗能够通过促进癌细胞凋亡、抑制癌细胞侵袭及血管新生的方式来降低肿瘤恶性程度。

Objective：To study the effect of paclitaxel＋cisplatin chemotherapy before radical operation for esophageal cancer on the malignant degree of tumor.Methods：Patients who underwent radical operation for esophageal cancer in Zigong Third People＇s Hospital between March 2015 and November 2017 were selected and divided into experimental group and control group according to preoperative application of paclitaxel＋cisplatin neoadjuvant chemotherapy or not in the history data.After surgery,right amount of esophageal cancer tissue was collected from the two groups of patients to extract RNA and then determine the mRNA expression of apoptosis genes Smac,Caspase-9,p21 and Spink8,invasion genes ADAMTS5,CNTN1,β-catenin,MMP9 and N-cadherin as well as angiogenesis molecules TGF-β1 and VEGF.Results：Smac,Caspase-9,p21 and Spink8 mRNA expression in surgically removed esophageal cancer tissues of experimental group were significantly higher than those of control group,ADAMTS5,CNTN1,β-catenin,MMP9 and N-cadherin mRNA expression in surgically removed esophageal cancer tissues were significantly lower than those of control group,and TGF-β1 and VEGF levels in serum as well as TGF-β1 and VEGF mRNA expression in surgically removed esophageal cancer tissues were significantly lower than those of control group.Conclusion：paclitaxel＋ cisplatin chemotherapy before radical operation for esophageal cancer can reduce the malignant degree of tumor by promoting the cancer cell apoptosis and inhibiting the cancer cell invasion and angiogenesis.