Short-term Effects of Nitrogen Deposition on Soil Enzyme Activities in Tibet Grasslands

[Objective] The paper was to study the effects of nitrogen deposition on enzyme activity in different layers of soil. [Method] With grass-land located in Zhuqudeng Village, Bujiu Township, Linzhi City, the Tibet Autonomous Region, as the object, nitrogen deposition was simulated from July 2014 to August 2015. Four N addition treatments were established： control （0 kg·hm^2/a）, low N （LN, 25kg·hm^2/a）, medium N （MN, 50 kg·hm^2/a） and High N （HN, 150 kg·hm^2/a）, aiming at measuring enzyme activity （invertase, catalase, urease, amylase, cellulase, polyphenol oxidase and β-glucosi- dase） in different layers of grassland soil （0 -20 cm and 20-40 cm）. [Result] （1） Different levels of simulated nitrogen deposition had significant impact on invertase and β-glucosidase at the soil depth of 0-20 cm （P〈0.05）, but no significant impact on catalase, urease, amylase, cellulase and polyphenol oxidase（P〉0.05）; invertase, polyphenol oxidase and β-glucosidase had significant response to nitrogen deposition at the soil depth of 20- 40 cm （P〈0.05）, while catalase, urease, amylase and cellulose had no significant response （P〉0.05）. （2） The activities of invertase and polyphenol oxidase were enhanced at the soil depth of 0-20 cm, and that of β-glucosidase was inhibited. （3） With the deepening of nitrogen deposition, the ac- tivities of invertase and cellulose were enhanced at the soil depth of 20-40 cm; the activity of polyphenol oxidase was reduced in LN treatment, but increased in HN treatment; the activity of β-glucosidase was increased in LN treatment, but decreased in MN treatment. （4） With the deepening of soil layers, invertase and polyphenol oxidase responded similarly to simulated nitrogen deposition. [Conclusion] The results provide certain scientific basis and fundamental data for better understanding and evaluating the effects of nitrogen deposition on enzyme activity in grassland soil.